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Cd4 fitc

Manufactured by Southern Biotech
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CD4-FITC is a fluorescent-labeled antibody that binds to the CD4 cell surface receptor. It is used for the identification and quantification of CD4-positive cells in flow cytometry applications.

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Lab products found in correlation

Cd3 sprd, by Southern Biotech (2 mentions) Cd8 pe, by Southern Biotech (1 mentions) Igg1 pe, by Southern Biotech (1 mentions) Igg1 fitc, by Southern Biotech (1 mentions) Amnis flowsight imaging flow cytometer, by Merck Group (1 mentions) Cd8α fitc, by Southern Biotech (1 mentions) Cellquest, by BD (1 mentions) Cd8 rpe, by Southern Biotech (1 mentions) Facscalibur, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Anti chicken cd3 fitc, by Southern Biotech (1 mentions) Cd8a pe, by Southern Biotech (1 mentions)

Close spelling variants of this product

Mouse anti-chicken CD4-FITC FITC)-conjugated mouse anti-chicken CD4 antibody FITC-conjugated mouse anti-chicken CD4

4 protocols using cd4 fitc

1

Broiler's Intestinal Immune Profiling

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The cellular mucosal immune system status of the broiler's intestinal tract, intraepithelial lymphocytes (CD3+ T lymphocytes, CD3+CD8+ cells, CD3+CD4+ cells, and CD3+CD4+CD8 cells) were measured. At post mortem, intraepithelial T lymphocytes were estimated from isolated jejunum of 40 birds (1 bird per cage) at 42 d of age according to the method defined by Röhe (2014) . The intestinal cell suspensions were stained with either a cocktail of T lymphocyte CD marker antibodies (CD3-AF647, CD4-FITC and CD8-PE; Southern Biotech, Birmingham, AL, USA) or a cocktail of isotype control antibodies (IgG1-PE, IgG1-FITC, and IgG1-AF647; Southern Biotech, Birmingham, AL, USA) for 30 min on ice in the dark according to the manufacturer's instructions.
Flow cytometric data were acquired on an Amnis FlowSight imaging flow cytometer (Millipore, Burlington, Massachusetts, USA). CD4-FITC and CD8-PE were detected using the 488 nm laser, and the CD3-AF647 was detected using the 633 nm laser. Signals from the isotype antibody cocktail were subtracted from the T lymphocyte CD antibody fluorescence. From the total cell population that was acquired, the CD3+ intact cell population was gated. Sub-gates were applied to the intact CD3+ cells population to determine the proportion of cytotoxic T lymphocytes (CD3+CD8+), T helper lymphocyte (CD3+CD4+) and double-stained T lymphocytes (CD4+CD8+).
de Souza Vilela J., Andronicos N.M., Kolakshyapati M., Hilliar M., Sibanda T.Z., Andrew N.R., Swick R.A., Wilkinson S, & Ruhnke I. (2021). Black soldier fly larvae in broiler diets improve broiler performance and modulate the immune system. Animal Nutrition, 7(3), 695-706.
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2

Multiparametric Flow Cytometry Immunophenotyping

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Cells were diluted in staining buffer (PBS 1×, 10% FBS, 0.1% Sodium Azide) and 1 × 106 cells per well were transferred into 96 well-plates (V-shape), and washed twice with the same buffer. Staining was performed by resuspending the cellular pellet of each well with 100 μL of staining buffer including different combinations of antibodies, or as single-color stainings for compensation. Cells were incubated at 4 °C for 30 min and washed twice with staining buffer by centrifugation at 250 × g for 5 min.
Avian monoclonal antibodies (mAbs) (CD3-SPRD, CD4-FITC, CD8α-PE, CD8α-FITC and CD8β-PE) were purchased from Southern Biotech (Birmingham, AL, USA). Mononuclear cell suspensions from every thymus and spleen were stained with one and two mAb mix, respectively: CD3-SPRD, CD4-FITC and CD8α-PE in tube A (for thymocytes and splenocytes), and CD3-SPRD, CD8α-FITC and CD8β-PE in tube B (splenocytes). All antibodies were titrated in order to determine the optimal staining concentration of each one.
Positive cells were analyzed with a FACS Calibur flow cytometer (BD Biosciences, San Jose, CA, USA) and CellQuest software. Analysis was done on 20 000 events and discrete viable lymphoid cell populations were gated according to the forward/side scatter characteristics. Percentages of different lymphoid cell subpopulations in the thymus and spleen were determined by multiparametric analysis.
Rimondi A., Pinto S., Olivera V., Dibárbora M., Pérez-Filgueira M., Craig M.I, & Pereda A. (2014). Comparative histopathological and immunological study of two field strains of chicken anemia virus. Veterinary Research, 45(1), 102.
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3

Splenic Lymphocyte CD4+/CD8+ Ratio Analysis

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The splenic lymphocytes were collected for the measurement of the CD4+/CD8+ ratio as previously described27 (link). Briefly, 1 × 106 splenic lymphocytes were incubated with anti-chicken CD3-SPRD, CD4-FITC and CD8-RPE (Southern Biotech, USA) at 4 °C for 30 min. Subsequently, lymphocytes were washed 3 times with phosphate buffer saline containing 1% fetal bovine serum, then resuspended and analyzed by FacsCalibur and CellQuest software (Becton Dickinson, USA). Viable lymphocytes were calculated based on light scatter (forward and side scatter) characteristics, and 10,000 events were analyzed for positive staining with SPRD, FITC and RPE antibodies.
Chu J., Zhang Q., Zuo Z., El-Ashram S., Guo Y., Zhao P., Huang S., He C, & Khan A. (2017). Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens. Scientific Reports, 7, 13997.
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4

Ileal Lymphocyte Subset Analysis

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The ileal LPLs were isolated and purified and lymphocyte subpopulations were determined as described previously. 23, 24 Briefly, the isolated lymphocytes were stained using antichicken CD3-FITC, CD4-FITC, CD8a-PE, and BU-1-FITC from Southern Biotech Associates (Birmingham, AL) and incubated for 30 min at 37 °C, then washed with PBS twice. A FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ) was used to measure the proportions of CD3 + , CD4 + , CD8 + and B cells.
Gates were drawn around cells and a 2-parameter dot-plot histogram was used to analyze the fluorescence data collected on at least 50 000 lymphocytes. Cell phenotypic data are expressed as a percentage of gated lymphocytes.
Ruan D., Wu S., Fouad A.M., Zhu Y., Huang W., Chen Z., Gou Z., Wang Y., Han Y., Yan S., Zheng C, & Jiang S. (2022). Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens. Food & function, 13(22).
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