Anti cd49f apc

Manufactured by Thermo Fisher Scientific

Anti-CD49f-APC is a fluorescently-labeled antibody that binds to the CD49f antigen. CD49f is a cell surface protein that is expressed on various cell types, including stem cells and certain cancer cells. The Anti-CD49f-APC antibody can be used to detect and analyze cells expressing CD49f in flow cytometry applications.

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Lab products found in correlation

Calibur, by BD (1 mentions) Anti epcam apc, by Thermo Fisher Scientific (1 mentions) Anti sca 1 pe, by Thermo Fisher Scientific (1 mentions) Anti cd34 pe, by Thermo Fisher Scientific (1 mentions) Anti cd44 pe, by Thermo Fisher Scientific (1 mentions) Anti α6 integrin apc, by Thermo Fisher Scientific (1 mentions) Anti cd45 apc, by Thermo Fisher Scientific (1 mentions) Anti epcam fitc, by BioLegend (1 mentions) Lsrii cytometer, by BD (1 mentions)

2 protocols using anti cd49f apc

Characterizing OC Stem Cell Markers

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Primary isolated OC were cultured in vitro and passaged for about 6-8 times. The OCs were harvested and then incubated with anti-EpCAM-APC, anti-Sca-1-PE, anti-CD44-PE, anti-CD49f-APC, anti-CD45-APC, anti-CD34-PE, anti-c-kit-FITC, anti-α6 integrin-APC, anti-β1 integrin-PercpCy5.5, anti-β4 integrin-PercpCy5.5 (all from ebioscience, San Diego, CA) at 4°C for 30 mins, after that, cells were fixed with 1% paraformaldehyde and analyzed by a BD Calibur (Becton, Dickinson and Company, New Jersey) and Flowjo software (Tree Star, San Carlos, CA).

Qian L., Zhang H., Gu Y., Li D., He S., Wang H., Cheng Y., Yang W., Yu H., Zhao X., Cai W., Meng L., Jin M., Wang Y, & Zhang Y. (2018). Reduced production of laminin by hepatic stellate cells contributes to impairment in oval cell response to liver injury in aged mice. Aging (Albany NY), 10(12), 3713-3735.

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Immunophenotyping Mammary Progenitor Cells

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Anti-CD21-PE antibody (HB5, eBioscience) was used for CD21 detection. Anti-CD24-PE and anti CD44-APC antibodies (eBioscience) were used to detect progenitor cells. Anti-CD49f–APC (eBioscience) and anti-EpCAM-FITC (Biolegend) antibody were used for detection of mammary epithelial lineage. Isotype matched antibodies were used as negative controls. Flow cytometry was performed on a LSRII cytometer (BD Biosciences) and data were analyzed with FlowJo.

Hu H., Luo M.L., Desmedt C., Nabavi S., Yadegarynia S., Hong A., Konstantinopoulos P.A., Gabrielson E., Hines-Boykin R., Pihan G., Yuan X., Sotirious C., Dittmer D.P., Fingeroth J.D, & Wulf G.M. (2016). Epstein–Barr Virus Infection of Mammary Epithelial Cells Promotes Malignant Transformation. EBioMedicine, 9, 148-160.

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