Sorafenib no s7397

Male BALB/c nu/nu mice (6–8 weeks old, Shanghai Institute of Material Medicine, Chinese Academy of Science) were housed in specific pathogen-free conditions. All animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” prepared by the National Academy of Sciences and published by the National Institutes of Health (NIH publication 86–23 revised 1985). Sorafenib (No. S7397), Rapamycin (No. S1039), and Metformin (No. S1950) were purchased from Selleck Chemicals (Houston, TX, USA). The PI (propidiumiodide)/Annexin V-FITC apoptosis detection kit was from BD Biosciences (San Jose, CA, USA). The Cell Counting Kit-8 (CCK-8) kit was purchased from Dojindo Laboratories (Kumamoto, Japan). Edu assays (KGA331-100) were purchased from KeyGENE BioTECH (Nanjing, China).

Antibodies to NRF2 (ab62352) and GPX4 (ab125066), as well as necrosulfonamide (ab143839), were obtained from Abcam (Shanghai, China). Antibody to S1R (sc137075) was obtained from Santa Cruz (Shanghai, China). Erastin (No. S7242), sorafenib (No. S7397), ZVAD‐FMK (No. S7023) and ferrostatin‐1 (No. S7243) were obtained from Selleck (Shanghai, China). All‐trans retinoic acid (ATRA; R2625), trigonelline (T5509), PRE‐084 (P2607), BD1063 (SML0276) and BD1047 (B8562) were obtained from Sigma‐Aldrich (Shanghai, China).