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Dcdms

Manufactured by Merck Group
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DCDMS is a laboratory equipment product manufactured by Merck Group. It is a reagent used in various chemical and analytical procedures. DCDMS stands for Dichlorodimethylsilane, and it is a colorless, flammable liquid with a pungent odor. The core function of DCDMS is to act as a silylating agent, which is used to introduce silyl groups into organic compounds.

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Lab products found in correlation

Temed, by Merck Group (4 mentions) Bis acrylamide, by Merck Group (2 mentions) St005, by Beyotime (2 mentions) Bis acrylamide, by Sangon (2 mentions) Glass coverslip, by Avantor (1 mentions) Glutaraldehyde solution, by Thermo Fisher Scientific (1 mentions) Triton x 100 detergent, by Merck Group (1 mentions) Acrylamide, by Merck Group (1 mentions) Aptes, by Merck Group (1 mentions) Sodium hydrogen carbonate, by Merck Group (1 mentions) Analar normapur, by Avantor (1 mentions)

6 protocols using dcdms

1

Polyacrylamide Gel Preparation for AFM

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Solutions of acrylamide and bis-acrylamide in PBS were polymerized with 10% ammonium persulfate and TEMED (Sigma-Aldrich, USA). Concentrations of acrylamide (10%) and bis-acrylamide (0.01%, w/v) were chosen to prepare a PAAm gel with approximately 1–2 kPa Young’s modulus, which is close to the eukaryotic cell stiffness. For AFM experiments, a total solution volume of 75 μL was allowed to polymerize between circular 18 mm (top) and square 24 mm (bottom) glass coverslips (VWR, USA). The bottom coverslip was aminosilanized with 3-aminopropyltriethoxysilane (APTES, Sigma-Aldrich, USA) and further coated with 0.5 (v/v)% glutaraldehyde solution in PBS (Fischer Scientific, USA) for at least 30 minutes. The upper coverslip was silanized with dichlorodimethylsilane (DCDMS, Sigma-Aldrich, USA). After 30 minutes of polymerization, the top coverslip was removed and the sample was extensively washed with PBS. Prepared PAAm gels had a ~200 μm thickness. The PAAm gels were kept in PBS for several days before the experiments to reach equilibrium. AFM measurements were performed in PBS containing 0.1% Triton X-100 detergent (Sigma-Aldrich, USA) to decrease probe-gel adhesion.
Efremov Y.M., Wang W.H., Hardy S.D., Geahlen R.L, & Raman A. (2017). Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves. Scientific Reports, 7, 1541.
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2

Fabrication of Polyacrylamide Gels

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Polyacrylamide gels with two different crosslinking densities were prepared by mixing acrylamide and bis-acrylamide (Sigma-Aldrich, USA) stock solutions at different concentrations as summarized in Table 1. The details of the preparation of polyacrylamide gels are given in ref. 53 . Briefly, glass slides, coverslips, and glass boats (vials) were piranha cleaned (70% H2SO4 and 30% H2O2) for 30 minutes at 80 °C, extensively rinsed with DI water, and then dried in a stream of dry nitrogen. The coverslips were then placed in a glass jar and were silanized via vapour deposition using 1 mL of 3-aminopropyltriethoxysilane (APTES) (Sigma-Aldrich, USA) for 2 hours at 80 °C inside a preheated oven. Silanized coverslips were placed in a glass jar containing 0.5 (v/v)% glutaraldehyde solution in PBS solution (Ca2+ and Mg2+ free, 0.016 M, Fischer, USA) for at least 30 minutes. The glass slides and the boats were placed in a glass jar containing 1 mL of dichlorodimethylsilane (DCDMS) (Sigma-Aldrich, USA) and were silanized for 45 minutes under a continuous nitrogen flow through the jar. Finally, radical polymerization was initiated between the monomer and the crosslinker solution (2 mL) by adding tetramethylethylenediamine (TEMED) (2 μL) (Sigma-Aldrich, USA) and ammonium persulfate (APS) (20 μL, 10 w/v% in PBS) (Sigma-Aldrich, USA) in an eppendorf tube.
Nalam P.C., Gosvami N.N., Caporizzo M.A., Composto R.J, & Carpick R.W. (2015). Nano-rheology of hydrogels using direct drive force modulation atomic force microscopy. Soft matter, 11(41), 8165-8178.
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3

Synthesis of Polyborosiloxane Polymer

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In order to synthesize the polymer, one part of dichlorodimethylsilane (DCDMS)(Sigma-Aldrich) was dissolved in two parts of diethyl ether anhydrous (VWR AnalaR NORMAPUR®) and hydrolyzed with two parts of distilled water. The produced polydimethilsiloxane (PDMS) was washed in a saturated solution of sodium hydrogencarbonate (Sigma-Aldrich) and the residual solvent evaporated. Polyborosiloxane (PBS) was obtained by the heat-assisted reaction of PDMS with boron oxide nanoparticles (B2O3 SkySpring Nanomaterials,Inc, average size 80 nm). The final product was dissolved in ethanol with a ratio of 80 vol% PBS : 20 vol% Ethanol.
D’Elia E., Eslava S., Miranda M., Georgiou T.K, & Saiz E. (2016). Autonomous self-healing structural composites with bio-inspired design. Scientific Reports, 6, 25059.
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4

Polyacrylamide Hydrogel Fabrication

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As shown in Supplementary Table 1, the concentrations of acrylamide and bis-acrylamide (79-06-1, 110-26-9, Sangon, Shanghai, China) were altered to establish polyacrylamide hydrogels of different stiffness as described previously [31 (link)]. In brief, the coverslips were incubated with 3-aminopropyltrimethoxysilane (APES, 919-30-2, Solarbio, Beijing, China), and glass slides were treated with dichlorodimethylsilane (DCDMS, 08471, Sigma-Aldrich, Burlington, Massachusetts). Acrylamide, bis-acrylamide and 0.1% TEMED (110-18-9, Sigma-Aldrich) were mixed with 1% ammonium persulfate (ST005, Beyotime) and then added onto glass slides and covered by coverslips before polymerization. Ten minutes later, the hydrogels were removed from the slides and placed into 6-well-plates for cell culture.
Zhou Z., Suo Y., Bai J., Lin F., Gao X., Shan H., Ni Y., Zhou X., Sheng L, & Dai J. (2023). Matrix Stiffness Activating YAP/TEAD1-Cyclin B1 in Nucleus Pulposus Cells Promotes Intervertebral Disc Degeneration. Aging and Disease, 14(5), 1739-1756.
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5

Hydrogel Substrate Preparation Protocol

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All procedures for the preparation of hydrogel substrates were described in a previous study [33 ]. In brief, 25-mm coverslips were coated with 0.1 M NaOH and APES (Sigma). The coverslips were washed with distilled H2O and then incubated in 0.5% glutaraldehyde for 30 min. Glutaraldehyde was removed, and the coverslips were air dried. Chloro-silanated glass slides were prepared by coating them with DCDMS (Sigma) and washing with distilled H2O. The gel mixture was prepared by mixing acrylamide and bis-acrylamide in the desired ratio. After the addition of a 1/100 total volume of APS and a 1/1000 total volume of TEMED to the gel mixture, 25 μL of gel solution was placed on a chloro-silanated glass slide and covered with an amino-silanated coverslip. After polymerization, the bottom glass slide was removed, and the gel-coverslip composite was placed in a 6-well plate and washed with distilled H2O.
Hwang J.H., Byun M.R., Kim A.R., Kim K.M., Cho H.J., Lee Y.H., Kim J., Jeong M.G., Hwang E.S, & Hong J.H. (2015). Extracellular Matrix Stiffness Regulates Osteogenic Differentiation through MAPK Activation. PLoS ONE, 10(8), e0135519.
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6

Polyacrylamide Hydrogel Fabrication

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As shown in Supplementary Table 1, the concentrations of acrylamide and bis-acrylamide (79-06-1, 110-26-9, Sangon, Shanghai, China) were altered to establish polyacrylamide hydrogels of different stiffness as described previously [31] . In brief, the coverslips were incubated with 3-aminopropyltrimethoxysilane (APES, 919-30-2, Solarbio, Beijing, China), and glass slides were treated with dichlorodimethylsilane (DCDMS, 08471, Sigma-Aldrich, Burlington, Massachusetts). Acrylamide, bis-acrylamide and 0.1% TEMED (110-18-9, Sigma-Aldrich) were mixed with 1% ammonium persulfate (ST005, Beyotime) and then added onto glass slides and covered by coverslips before polymerization. Ten minutes later, the hydrogels were removed from the slides and placed into 6-well-plates for cell culture.
Zhou Z., Suo Y., Bai J., Lin F., Gao X., Shan H., Ni Y., Zhou X., Sheng L, & Dai J. (2023). Matrix Stiffness Activating YAP/TEAD1-Cyclin B1 in Nucleus Pulposus Cells Promotes Intervertebral Disc Degeneration. Aging and disease.
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