Ham s f12 mcdb 105

Three human thyroid cancer cell lines FTC-133 (Sigma-Aldrich, Italy), K1 (Sigma-Aldrich, Italy), and 8505c (Sigma-Aldrich, Italy) were used in this study. FTC-133 cells were cultured in DMEM: Ham’s F12 (1:1) (Sigma-Aldrich, Italy) supplemented with L-Glutamine 2 mM (Euroclone, Italy), penicillin/streptomycin/amphotericin (PSA) (Euroclone, Italy), and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich, Italy). K1 cells were cultured in DMEM: Ham’s F12: MCDB 105 (2:1:1) (Sigma-Aldrich, Italy) supplemented with L-Glutamine 2 mM (Euroclone, Italy), penicillin/streptomycin/amphotericin (PSA) (Euroclone, Italy), and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich, Italy), while for 8505c cells, the medium employed was EMEM (Sigma-Aldrich, Italy) containing 1% of nonessential amino acids, L-Glutamine 2 mM, PSA, and 10% FBS. Cells were maintained in a humidified environment at 37°C and 5% CO₂/95% air atmosphere and cultured in 75 cm² culture flasks. The medium was replaced twice a week and cells were split at about 80–90% of the confluence.