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Bistris bolt mini gel

Manufactured by Thermo Fisher Scientific
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The BisTris Bolt mini gel is a pre-cast polyacrylamide gel designed for the separation of proteins. It utilizes a BisTris buffer system and is suitable for use in mini gel electrophoresis systems. The gel dimensions are 8 cm x 8 cm with a thickness of 1 mm.

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Lab products found in correlation

Ab30871, by Abcam (2 mentions) Ab19903, by Abcam (2 mentions) Ab77581, by Santa Cruz Biotechnology (2 mentions) Ab217345, by Abcam (2 mentions) A16110, by Thermo Fisher Scientific (2 mentions) Epr15314 ab186429, by Abcam (2 mentions) Epr21151, by Abcam (2 mentions) A18745, by Thermo Fisher Scientific (2 mentions) Pierce elc western substrate, by Thermo Fisher Scientific (2 mentions) Ai600 imager, by GE Healthcare (2 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (2 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions)

2 protocols using bistris bolt mini gel

1

Western Blot Analysis of Extracellular Vesicles

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Cells and 100,000 g cell pellets were lysed in RIPA buffer (10mM Tris-HCL, 1mM EDTA, 0.5mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 140mM NaCl) with 1mM PMSF for 15min on ice. Protein was quantified using a Pierce BCA Protein Assay (Thermo Scientific). Lysates were resolved on a 4%–12% BisTris Bolt mini gel (Invitrogen) and transferred to nitrocellulose membrane. Membranes were blocked with 5% powdered milk or 5% BSA and washed with TBS-T (0.1% Tween-20, 137mM NaCl, 20mM Tris Base). Primary antibodies were used at manufacturer recommended dilutions and included anti-Alix (Abcam, EPR15314 ab186429, RRID: AB_2754981), anti-Tsg101 (Abcam, ab30871, RRID: AB_2208084), anti-CD63 (Abcam, EPR21151, ab217345, RRID: AB_2754982), anti-Syntenin (Abcam, ab19903, RRID: AB_445200), anti-Arf6 (Abcam, ab77581, RRID: AB_2058475), and anti-CD9 (Santa Cruz, KMC8.8, sc-18869, RRID: AB_2076043). Secondary HRP antibodies were goat anti-rabbit (Invitrogen, A16110, RRID: AB_2534782) and donkey anti-rat (Invitrogen, A18745, RRID: AB_2535522), and blots detected with Pierce ELC Western Substrate (Thermo) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo) on an AI600 imager (GE Healthcare). Bands were quantified using ImageJ, RRID:SCR_003070.
Pua H.H., Happ H.C., Gray C.J., Mar D.J., Chiou N.T., Hesse L.E, & Ansel K.M. (2019). Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses. Cell reports, 26(4), 933-944.e4.
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2

Western Blot Analysis of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells and 100,000 g cell pellets were lysed in RIPA buffer (10mM Tris-HCL, 1mM EDTA, 0.5mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 140mM NaCl) with 1mM PMSF for 15min on ice. Protein was quantified using a Pierce BCA Protein Assay (Thermo Scientific). Lysates were resolved on a 4%–12% BisTris Bolt mini gel (Invitrogen) and transferred to nitrocellulose membrane. Membranes were blocked with 5% powdered milk or 5% BSA and washed with TBS-T (0.1% Tween-20, 137mM NaCl, 20mM Tris Base). Primary antibodies were used at manufacturer recommended dilutions and included anti-Alix (Abcam, EPR15314 ab186429, RRID: AB_2754981), anti-Tsg101 (Abcam, ab30871, RRID: AB_2208084), anti-CD63 (Abcam, EPR21151, ab217345, RRID: AB_2754982), anti-Syntenin (Abcam, ab19903, RRID: AB_445200), anti-Arf6 (Abcam, ab77581, RRID: AB_2058475), and anti-CD9 (Santa Cruz, KMC8.8, sc-18869, RRID: AB_2076043). Secondary HRP antibodies were goat anti-rabbit (Invitrogen, A16110, RRID: AB_2534782) and donkey anti-rat (Invitrogen, A18745, RRID: AB_2535522), and blots detected with Pierce ELC Western Substrate (Thermo) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo) on an AI600 imager (GE Healthcare). Bands were quantified using ImageJ, RRID:SCR_003070.
Pua H.H., Happ H.C., Gray C.J., Mar D.J., Chiou N.T., Hesse L.E, & Ansel K.M. (2019). Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses. Cell reports, 26(4), 933-944.e4.
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