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Hema 3 system

Manufactured by Thermo Fisher Scientific
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The Hema 3 System is a hematology analyzer designed for clinical laboratory use. The device performs automated analysis of complete blood count (CBC) parameters, including red blood cells, white blood cells, and platelets. The Hema 3 System provides accurate and reliable results to support clinical decision-making.

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Lab products found in correlation

Axiovert microscope, by Zeiss (4 mentions) Bx43f, by Olympus (1 mentions) Cytospin 2, by Thermo Fisher Scientific (1 mentions) Evos fl auto imaging system, by Thermo Fisher Scientific (1 mentions) Facsaria fusion flow cytometer, by BD (1 mentions) Sf100 4, by Thermo Fisher Scientific (1 mentions) Facsaria 2, by BD (1 mentions) Ficoll, by GE Healthcare (1 mentions)

Close spelling variants of this product

Hema 3 (99 citations) Hema 3 Manual Staining System (12 citations) Hema-3 staining system (10 citations) PROTOCOL Hema 3 Manual Staining System (4 citations) Hema-3 Stain System (3 citations)

15 protocols using hema 3 system

1

siRNA, qPCR, and Cell Migration Assays

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siRNA, qPCR and growth assays were performed as previously described [47 (link)]. qPCR sequences are listed in Supplementary Table S6. Migration and invasion assays were performed as previously reported [48 (link)], allowing 18 hours for migration, after which passed cells were stained (Hema 3 System, Fischer Scientific #22-122911) and five 20x fields counted.
Shepherd J.H., Uray I.P., Mazumdar A., Tsimelzon A., Savage M., Hilsenbeck S.G, & Brown P.H. (2016). The SOX11 transcription factor is a critical regulator of basal-like breast cancer growth, invasion, and basal-like gene expression. Oncotarget, 7(11), 13106-13121.
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2

Cell Cytospin Staining and Imaging

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For cytospin, purified cells were spun onto glass slides, fixed and stained using the Hema 3 System (Thermo Fisher Scientific), and rinsed in distilled water. Images were analyzed using an Olympus BX43F conjugated with an Olympus DP21 digital camera. Snapshots were taken at a 10 × 100-fold magnification.
Almeida F.F., Tenno M., Brzostek J., Li J.L., Allies G., Hoeffel G., See P., Ng L.G., Fehling H.J., Gascoigne N.R., Taniuchi I, & Ginhoux F. (2015). Identification of a novel lymphoid population in the murine epidermis. Scientific Reports, 5, 12554.
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3

Immune Cell Profiling in Lavage Samples

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Lavage cells were cytocentrifuged (Cytospin 2, Thermo Fisher Scientific, Waltham, MA) and stained using a Hema3 System (Thermo Fisher Scientific). Cell differential percentages were determined by light microscopic evaluation of Hema3-stained cytospin preparations and expressed as absolute cell numbers. IL-4 and IFN-γ were measured using MesoScale Discovery Mouse V-Plex Pro-Inflammatory Panel 1 assay (Rockville, MD). IL-13 was measured using a sensitive Quantikine Mouse IL-13 immunoassay Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. Samples were carried out in two separate batches for this assay.
Lee J.W., Jaffar Z., Pinkerton K.E., Porter V., Postma B., Ferrini M., Holian A., Roberts K, & Cho Y.H. (2015). Alterations in DNA methylation and airway hyperreactivity in response to in utero exposure to environmental tobacco smoke. Inhalation toxicology, 27(13), 724-730.
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4

Allergic Airway Inflammation: Characterizing Cell Profiles

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Mice were sacrificed 24 h after the last allergen challenge. Total number of cells in the BALF was counted in a hemocytometer and differential cell counts in the BALF and blood were determined from cytocentrifuged samples based on morphologic and histologic criteria after staining with Hema 3 System (Thermo Fisher Scientific). BALF supernatants were stored at −80°C for later analysis. Right lungs were snap-frozen and left lungs were first perfused with 4% paraformaldehyde (PFA), fixed in 4% PFA and paraffin embedded. Lung tissue harvested from WT C57BL/6 mice challenged with A. alternata (22 (link)), ovalbumin (23 (link)) or cockroach allergen (24 (link)) and paraffin embedded in a manner similar was used in some studies.
Dileepan M., Ge X.N., Bastan I., Greenberg Y.G., Liang Y., Sriramarao P, & Rao S.P. (2019). Regulation of eosinophil recruitment and allergic airway inflammation by tropomyosin receptor kinase A. Journal of immunology (Baltimore, Md. : 1950), 204(3), 682-693.
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5

Quantifying Lung Inflammatory Responses to MWCNT

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To determine the lung's inflammatory responses to MWCNT aspiration, we performed differential scoring of the cells recovered in the first BAL. The cells in a 100-μl aliquot of the first lavage were centrifuged (Cytospin 3, Shandon Life Sciences International Ltd., Cheshire, England) and then differentially stained using the Hema 3™ system (Thermo Fisher Scientific, Waltham, MA), a modification of the Wright-Giemsa method. The percentage of specific inflammatory cell types (eosinophils, lymphocytes, macrophages/monocytes, and neutrophils) was determined by counting at least 500 cells from each mouse. We report the percentage of cell types instead of the total number, because a multi-center study using standardized protocols demonstrated significant interlaboratory variation in the total number of recovered cell types, despite standardization [2] (link).
Cartwright M.M., Schmuck S.C., Corredor C., Wang B., Scoville D.K., Chisholm C.R., Wilkerson H.W., Afsharinejad Z., Bammler T.K., Posner J.D., Shutthanandan V., Baer D.R., Mitra S., Altemeier W.A, & Kavanagh T.J. (2016). The pulmonary inflammatory response to multiwalled carbon nanotubes is influenced by gender and glutathione synthesis. Redox Biology, 9, 264-275.
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6

Allergen-Induced Tissue Responses

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At the end of the study, blood, bone marrow (BM) and jejunum were collected from allergen-challenged and control mice. Blood and BM were used to determine differential cell counts from cytocentrifuged samples based on morphologic and histologic criteria after staining with the Hema 3 System (Thermo Fisher Scientific Co., Pittsburgh, PA). Serum was used to determine IgE levels. The jejunum was divided into two segments. One segment was perfused with saline and snap-frozen in liquid nitrogen while the other was perfused and fixed in 4% (vol/vol) paraformaldehyde for 24 h before paraffin embedding.
Bastan I., Ge X.N., Dileepan M., Greenberg Y.G., Guedes A.G., Hwang S.H., Hammock B.D., Washabau R.J., Rao S.P, & Sriramarao P. (2018). Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation. Journal of leukocyte biology, 104(1), 109-122.
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7

Pulmonary Inflammation Assessment in Mice

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At the end of the study, lungs of mice were lavaged with 1 ml saline. Total and differential cell counts in the bronchoalveolar lavage fluid (BALF) were determined based on morphologic and histologic criteria after staining cytocentrifuged samples with Hema 3 System (Thermo Fisher Scientific, Pittsburgh, PA). BALF supernatants were stored at −80°C for later analysis. Right lungs were snap-frozen and left lungs were perfused with 4% paraformaldehyde (PFA) to preserve pulmonary structure, fixed in 4% PFA and paraffin embedded. Bone marrow (BM) was collected and used to determine eosinophil counts based on cell morphology after Hema 3 staining.
Dileepan M., Rastle-Simpson S., Greenberg Y., Wijesinghe D.S., Kumar N.G., Yang J., Hwang S.H., Hammock B.D., Sriramarao P, & Rao S.P. (2019). Effect Of Dual sEH/COX-2 Inhibition on Allergen-Induced Airway Inflammation. Frontiers in Pharmacology, 10, 1118.
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8

Flow Cytometry Analysis of Peritoneal Cells

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Peritoneal macrophages and DCs were sorted using a FACSAria Fusion flow cytometer (BD), concentrated by Cytospin, and stained with Wright–Giemsa stain using the Hema 3 system (Thermo Fisher Scientific). Bright-field images were captured at room temperature with a 40× objective lens using an EVOS FL Auto Imaging system (Invitrogen).
Wu X., Briseño C.G., Durai V., Albring J.C., Haldar M., Bagadia P., Kim K.W., Randolph G.J., Murphy T.L, & Murphy K.M. (2016). Mafb lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of Langerhans cells. The Journal of Experimental Medicine, 213(12), 2553-2565.
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9

Intranasal Influenza Infection in TrkA KI Mice

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A TrkAF592A knockin (TrkA KI) mouse line [36 (link)] was obtained from Dr. Keqiang Ye at Emory University and maintained in the research animal resources facility of University of Minnesota. Six-to 12-week-old mice were anesthetized by isoflurane and infected intranasally with 5xMLD50 of A/PR8 or A/CA04 in 50 μl volume. Animals were given either DMSO/PBS (16% v/v) or 1NMPP1 at 10 mg/kg/day by intramuscular (IM) or intravenous (IV) injection. In one study 1NMPP1 was given to mice in drinking water at 25 μM. Mice were monitored daily for clinical signs and body-weight loss up to day 21. Mice were euthanized if they reached pre-specified terminal points as previously described [50 (link)]. Mice were euthanized at specified time points. Bronchoalveolar lavage fluid (BALF) was collected for total and differentiated cell count after staining with Hema 3 System (Thermo Fisher Scientific). Upper and lower respiratory tracts, and right lungs were collected and homogenized for viral quantification by plaque assay. Left lungs were first perfused with 4% paraformaldehyde (PFA), fixed in 4% PFA and paraffin embedded for histopathological analysis.
Verma V., Dileepan M., Huang Q., Phan T., Hu W.S., Ly H, & Liang Y. (2022). Influenza A virus activates cellular Tropomyosin receptor kinase A (TrkA) signaling to promote viral replication and lung inflammation. PLoS Pathogens, 18(9), e1010874.
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10

Staining Peripheral Blood Smears

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Peripheral blood smears and cytospins from RBC lysed bone marrow samples were stained using the Hema 3 System (Fisher) following the manufacturer’s instructions. The images were acquired using a Zeiss Axiovert microscope with a digital camera.
Folgado-Marco V., Ames K., Chuen J., Gritsman K, & Baker N.E. (2023). Haploinsufficiency of the essential gene Rps12 causes defects in erythropoiesis and hematopoietic stem cell maintenance. eLife, 12, e69322.
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