Recombinant sars cov 2 spike protein

T cell responses were assessed using cytokine ELISA. Splenocytes from immunized mice were plated at a density of 5×10⁶ cells per well in 24-well plates. The cells were stimulated with 5 μg/mL recombinant SARS-CoV-2 Spike protein (ACROBiosystems) at 37°C for 3 days. The supernatant was harvested and assayed for cytokine production. Mouse IL-2, IL-5, IL-13 and IFN-γ were quantitated by ELISA using the matching antibody set (Invitrogen) in accordance with the manufacturer’s instructions.

T cell responses were assessed using cytokine ELISA. Splenocytes from immunized mice were plated at a density of 5 × 10⁶ cells per well in 24-well plates. The cells were stimulated with 5 μg/mL recombinant SARS-CoV-2 spike protein (ACROBiosystems, cat# SPN-C52H5) at 37 °C for 3 days. The supernatant was harvested and assayed for cytokine production. The levels of mouse secreted IFN-γ, IL-5, IL-13 and IL-2 were evaluated by ELISA using the matching antibody set (Invitrogen IFN-γ cat# 88-7314; IL-5 cat# 88-7054; IL-13 cat# 88-7137 and IL-2 cat# 88-7024-88) in accordance with the manufacturer’s instructions. Similarly, rat IFN-γ (Abnova, cat# KA3363), IL-13 (Abnova, cat# KA1408) and IL-4 (Invitrogen, cat# BMS628) will be quantitated by ELISA using the matching antibody set.