Anti cd9 ab92726

Manufactured by Abcam

Sourced in United States

Anti-CD9 (ab92726) is a mouse monoclonal antibody that recognizes the CD9 antigen. CD9 is a member of the tetraspanin family and is expressed on the surface of various cell types, including platelets, monocytes, and certain types of cancer cells. This antibody can be used for the detection and quantification of CD9 in research applications.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Anti parp sc 7150, by Santa Cruz Biotechnology (1 mentions) Anti alix ab186429, by Abcam (1 mentions) Anti gpc1pipa528055, by Thermo Fisher Scientific (1 mentions) Amt ccd camera, by Advanced Microscopy Techniques (1 mentions) Immobilon p, by Merck Group (1 mentions) Anti alix, by Santa Cruz Biotechnology (1 mentions) Non reducing laemmli sample buffer, by Bio-Rad (1 mentions) Amershan imager 600, by GE Healthcare (1 mentions) Ecl plus reagent, by GE Healthcare (1 mentions) Phosstop, by Merck Group (1 mentions) Hif 1α, by BD (1 mentions) Anti tsg101 ab83, by Abcam (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions)

Spelling variants of this product

Ab92726 (251 citations) Anti-CD9 (110 citations) CD9 (ab92726 (2 citations)

4 protocols using anti cd9 ab92726

Antibody Panel for Rab GTPase and Cell Markers

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Human anti-RAB5, RAB7, and RAB11 antibodies (Rab Family Antibody Sampler Kit #9385) were acquired from Cell Signaling Technology (Danvers, MA, USA); human anti-GAPDH (sc-47724), anti-Calnexin (sc-11397) and anti-PARP (sc-7150) antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA); human anti-Tsg101 (#MA1-23296) antibody was from Invitrogen (Carlsbad, CA, USA); anti-Alix (ab186429) and anti-CD9 (ab92726) antibodies were acquired from Abcam (Cambridge, UK).

Augimeri G., La Camera G., Gelsomino L., Giordano C., Panza S., Sisci D., Morelli C., Győrffy B., Bonofiglio D., Andò S., Barone I, & Catalano S. (2020). Evidence for Enhanced Exosome Production in Aromatase Inhibitor-Resistant Breast Cancer Cells. International Journal of Molecular Sciences, 21(16), 5841.

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Immunogold Labeling for Transmission Electron Microscopy

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Fixed specimens at an optimal concentration were placed onto a 400 mesh
carbon/formvar coated grids and allowed to absorb to the formvar for a minimum
of 1 minute. For immunogold staining the grids were placed into a blocking
buffer for a block/permeabilization step for 1 hr. Without rinsing, the grids
were immediately placed into the primary antibody at the appropriate dilution
overnight at 4°C (1:300 anti-CD9 ab92726, Abcam and anti-GPC1
PIPA528055, Thermo Scientific). As controls, some of the grids were not exposed
to the primary antibody. The following day, all the grids were rinsed with PBS
then floated on drops of the appropriate secondary antibody attached with 10 nm
gold particles (AURION, Hatfield, PA) for 2 hrs at room temperature. Grids were
rinsed with PBS and were placed in 2.5% Glutaraldehyde in 0.1M Phosphate
buffer for 15 min. After rinsing in PBS and distilled water the grids were
allowed to dry and stained for contrast using uranyl acetate. The samples were
viewed with a Tecnai Bio Twin transmission electron microscope (FEI, Hillsboro,
OR) and images were taken with an AMT CCD Camera (Advanced Microscopy
Techniques, Danvers, MA).

Melo S.A., Luecke L.B., Kahlert C., Fernandez A.F., Gammon S.T., Kaye J., LeBleu V.S., Mittendorf E.A., Weitz J., Rahbari N., Reissfelder C., Pilarsky C., Fraga M.F., Piwnica-Worms D, & Kalluri R. (2015). Glypican1 identifies cancer exosomes and facilitates early detection of cancer. Nature, 523(7559), 177-182.

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Western Blot Analysis of EV Proteins

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EVs or cells were lysed in 100 μL of RIPA buffer containing protease (Complete, Sigma-Aldrich) and phosphatase (PhosSTOP, Sigma-Aldrich) inhibitors. Equal amounts of protein samples were suspended in non-reducing Laemmli sample buffer (BioRad) and denatured at 100°C for 5 min. Proteins were separated on 10% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Immobilon-P; Millipore). Membranes were blocked with TBS containing 5% (w/v) nonfat dry milk powder with 0.1% Tween-20. Antibodies used were anti-CD9 (Ab 92726, Abcam), anti-TGS 101 (Santa Cruz. Sc-7964), anti-Alix (Santa Cruz. Sc-53538) and HIF-1α (610958, BD biosciences). Detection was carried out using peroxidase-conjugated secondary antibodies with the ECL Plus Reagent (Amersham, GE Healthcare, Munich, Germany). Proteins were visualized using an Amershan Imager 600 (GE Healthcare) and quantified with ImageJ software (NIH).

Ontoria-Oviedo I., Dorronsoro A., Sánchez R., Ciria M., Gómez-Ferrer M., Buigues M., Grueso E., Tejedor S., García-García F., González-King H., Garcia N.A., Peiró-Molina E, & Sepúlveda P. (2018). Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility. Frontiers in Cardiovascular Medicine, 5, 152.

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Protein Expression Analysis of Exosomes

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Tissue/cell/exosome samples were prepared by RIPA Lysis Buffer (Beyotime Biotechnology, China), and the protein concentration was measured by BCA Protein Assay Kit (Thermo Scientific, Somerset, NJ). Proteins were then separated by SDS‐PAGE (10%) and transferred to nitrocellulose membranes. After blocking with 5% fat‐free milk, the membranes were subsequently incubated with first antibodies at 4 °C including anti‐GM‐CSF (17762‐1‐AP, ProteinTech), anti‐GM130 (11308‐1‐AP, ProteinTech), anti‐TSG101 (ab83, Abcam), anti‐CD9 (ab92726, Abcam), and anti‐GAPDH (D110016‐0100, BBI Life Sciences). The membranes were washed three times in TBST to remove free peptides before incubation with the secondary antibodies (anti‐mouse [7076, CST] or anti‐rabbit [7074, CST]) in tris‐buffered saline at room temperature for 1 h. The protein bands were visualized with chemiluminescence (GE Healthcare, Chalfont St. Giles, UK). All the experiments were performed at least three times.

Ji P., Sun W., Zhang S., Xing Y., Wang C., Wei M., Li Q., Ji G, & Yang G. (2023). Modular Hydrogel Vaccine for Programmable and Coordinate Elicitation of Cancer Immunotherapy. Advanced Science, 10(22), 2301789.

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