Sheep polyclonal anti bubr1 antibody

A rabbit polyclonal anti-KIF2A antibody was purchased from Novus Biologicals (Littleton, CO, USA). Normal rabbit IgG was purchased from Santa Cruz Biotechnology (Dallas, CA, USA). A rabbit polyclonal anti-SKA1 antibody was purchased from Abcam (Cambridge, MA, USA). A rabbit monoclonal anti-TACC3 antibody was purchased from Abcam (Cambridge, MA, USA). Mouse monoclonal FITC-conjugated anti-α-tubulin antibody, TRITC-conjugated phalloidin, nocodazole and taxol were purchased from Sigma-Aldrich (St Louis, MO, USA). Sheep polyclonal anti-BubR1 antibody was purchased from Abcam (Cambridge, MA, USA). Alexa Fluor 546-conjugated donkey anti-sheep and donkey anti-rabbit antibodies were obtained from Invitrogen (Carlsbad, CA, USA). M2 medium was purchased from Sigma-Aldrich (St Louis, MO, USA). Milrinone was obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). Mounting medium containing DAPI was obtained from Vector Laboratories (Burlingame, CA, USA). Horseradish peroxidase (HRP)-conjugated mouse anti-rabbit IgG and horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG were obtained from Zhong Shan Jin Qiao Co. (Beijing, P. R. China). A mouse monoclonal anti-β-actin antibody was obtained from Abcam (Cambridge, MA, USA). Enhanced chemiluminescence (ECL) detection substrate was obtained from Xiamen Lulong Biotech Co. (Xiamen, P. R. China).

The CHK1 inhibitor Rabusertib was purchased from MedChemExpress. Sheep polyclonal anti‐BubR1 antibody, rabbit monoclonal anti‐γ‐H2A.X and anti‐MAP1LC3A antibodies were obtained from Abcam. The anti‐α‐tubulin‐FITC antibody and Hoechst 33342 were purchased from Sigma. AlexaFluor 488 goat anti‐rabbit antibody and AlexaFluor 594 goat anti‐rabbit antibody were obtained from Invitrogen. The human anti‐centromere CREST antibody was purchased from Fitzgerald Industries International. Mouse polyclonal anti‐BAX, rabbit polyclonal anti‐γ‐H2A.X antibody and anti‐Rad51 antibody were purchased from Proteintech. Rabbit monoclonal anti‐α‐tubulin antibody and phospho‐Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) were purchased from Cell Signaling Technology. FITC‐conjugated and TRITC‐conjugated goat anti‐rabbit IgG, TRITC‐conjugated goat anti‐mouse IgG and TRITC‐conjugated donkey anti‐human IgG were obtained from Zhongshan Golden Bridge Biotechnology, Co., Ltd. All other chemicals and reagents were from Sigma‐Aldrich Corp.

Rabbit polyclonal anti-Zfp207 antibody, sheep polyclonal anti-BubR1 antibody and mouse monoclonal anti-actin antibody were purchased from Abcam (Cambridge, MA, USA; Cat#: ab84802, ab28193 and ab3280); rabbit polyclonal anti-Bub3 antibody was purchased from Santa Cruz Biotechnology (Dallas, TA, USA; Cat#: sc-28258); mouse monoclonal anti-α-tubulin-fluorescein isothiocyanate (FITC) antibody and rabbit polyclonal anti-Bub1 antibody were purchased from Sigma (St. Louis, MO, USA; Cat#: F2168 and B3437); rabbit polyclonal anti-Mad2 antibody was purchased from Covance (Princeton, NJ, USA; Cat#: PRB-452C); Human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Davis, CA, USA; Cat#: 15-234).

Rabbit anti-ABCE1 monoclonal antibody (Cat# 185548) and sheep anti-BubR1 polyclonal antibody (Cat# 28193) were obtained from Abcam (Cambridge, UK); rabbit anti-phospho-p44/42 MAPK monoclonal antibody (Cat# 4370) was purchased from CST (Danvers, MA); mouse anti-γ-tubulin monoclonal antibody (Cat# 17787) was purchased from Santa Cruz (Santa Cruz, CA); mouse monoclonal anti-α-tubulin-FITC antibody (Cat# F2168) was obtained from Sigma (St Louis, MO). FITC-conjugated donkey anti-sheep IgG (H + L) was produced by Jackson ImmunoResearch Laboratories, Inc. Cy3-conjugated goat anti-rabbit IgG (H + L) and Cy3-conjugated goat anti-mouse IgG (H + L) were purchased from Boster Biotechnology Co., LTD (wuhan, China)
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Spindle perturbation drug nocodazole was 10 mg/ml in DMSO stock (−20°C), was purchased from Sigma-Aldrich Co. All other reagents were purchased from Sigma Aldrich unless specifically stated otherwise.

Mouse anti-Rae1 monoclonal antibody (Cat# SC-393252), mouse anti-Mad1 monoclonal antibody (Cat# 376613), rabbit anti-Cyclin B2 antibody (Cat# sc-2776), and mouse anti-securin monoclonal antibody (Cat# SC-56207) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse anti-α-tubulin-FITC antibody (Cat# F2168) was obtained from Sigma Chemical Company (St Louis, MO, USA); mouse anti-α-tubulin monoclonal antibody (66031-1-Ig) and mouse anti-β-actin monoclonal antibody (66009-1-Ig) were bought from Proteintech (Wuhan, China); rabbit anti-Cyclin B1 antibody (Cat# AF6168) was purchased from Affinity (Cincinnati, OH, USA); human anti-CREST antibody (Cat# 15-234-0001) was purchased from Antibodies Incorporated (Davis, CA, USA); sheep anti-BubR1 polyclonal antibody (Cat# 28193) was obtained from Abcam (Cambridge, UK); and DyLight 549-conjugated goat anti-mouse IgG (H + L) was purchased from Abbkine Biotechnology (California, CA, USA). FITC-conjugated donkey anti-sheep IgG (H + L) was produced by Jackson ImmunoResearch Laboratory (West Grove, PA, USA), FITC-conjugated goat anti-human IgG (H + L) was purchased from Boster (Wuhan, China), and TRITC-conjugated goat anti-human IgG (H + L) was purchased from Proteintech (Wuhan, China). All other chemicals and culture media were purchased from Sigma Chemical Company (St Louis, MO, USA), unless otherwise stated.