Invasion assay was performed as described previously47 (link). For this assay, 1 × 106 cells were cultured in the upper well of Matrigel chambers (Corning) containing serum-free medium and allowed to migrate towards serum-enriched medium in the bottom wells. After 20 h of incubation, invading or migrating cells were stained with 0.5% Giemsa, photographed with Nikon T1 E100 microscope, and counted using the Image J software program.
T1 e100 microscope
Manufactured by Nikon
The Nikon T1 E100 microscope is a compact, high-quality optical instrument designed for laboratory and research applications. It features a sturdy, ergonomic design and delivers reliable performance with its advanced optical system. The T1 E100 microscope is capable of magnifying specimens up to 1000x, making it suitable for a wide range of observation and analysis tasks.
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Lab products found in correlation
2 protocols using t1 e100 microscope
1
Matrigel Invasion Assay for Cell Migration
2
Wound Healing Assay for Cell Migration
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Migration assay was performed as described previously48 (link). Cells were plated in 6-well dish in triplicates and grew to 90% confluence and the cell surface was scratched with a sterile 20 µl tip. Images were captured with Nikon T1 E100 microscope at 0 h and post 24 h after the scratch to measure the wound healing by the cells, indicating their migratory capacity and migration rate. The images were analyzed using the Image J software program (National Institutes of Health; NIH), to measure the wound recovery by the cells indicating their migratory ability.
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