Td 08810

Mice were housed on a 12 h-light:dark cycle. CC founder strains were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) and were bred at University of Wisconsin, Madison. Mice were group housed by strain (2 mice/cage) and diet under a temperature- and humidity-controlled conditions, and received ad libitum access to water and food. After 4 weeks of age, mice were maintained on either a control (TD.08810, Envigo Teklad, 16.8%-kcal fat, 60.9% carbohydrate, 22.3% protein) or a high-fat high-sucrose diet (TD.08811, Envigo Teklad, 44.6%-kcal fat, 40.6% carbohydrate, 14.8% protein) (Table S1). Strains were housed within the same vivarium throughout the duration of the study.

In all studies, C57BL/6 male and female mice (Jackson Laboratory, Bar Harbor, ME, USA) were given water and chow ad libitum. High fat diet (HFD, 60% of calories from fat, TD.09766) and an isocaloric low fat diet (16.8% of calories from fat, TD.08810) were purchased from Envigo, Inc. (Indianapolis, IN, USA). For the HFD studies, six 6-week-old male mice were placed in each group and maintained on the diets for a total of 18 weeks. At 18 weeks colons were isolated, snap-frozen, and homogenized for analysis. All animal procedures were approved by the Ralph H Johnson VA Medical Center and Medical University of South Carolina Institutional Animal Care and Use Committees (IACUC) and all studies conducted were based on NIH and the American Veterinary Medical Association guidelines.