Prism 8.1.2 for mac

The criteria for statistical significance were defined as p < 0.05, and tests were performed for two-sided significance. Data was assessed for gaussian distribution using D’Agostino-Pearson normality testing, or Shapiro–Wilk testing for smaller sample size, with outlier analysis performed using the ROUT method. Parametric data is presented as a mean with 95% confidence intervals (95% CI) unless otherwise stated. Nonparametric data is presented as a median and inter-quartile range (IQR). Analysis was performed using either an unpaired t-test for parametric data, or the Mann–Whitney test for nonparametric data. Multiple variables with Gaussian distribution were compared using either one-way or two-way ANOVA, with post hoc testing performed on preselected pairs and corrected for multiple comparisons where indicated. Gene expression analysis was performed using Qbase + data analysis software, version 3.2 (Biogazelle, Zwijnaarde, Belgium), with results reported as mean values with standard error of the mean (SEM) based on an unpaired t-test. The inter-rater reliability of bone volume measurements across raters was determined using the intra-class correlation coefficient (ICC). Statistical analysis was performed except for gene expression data using Prism 8.1.2 for Mac (GraphPad Software, La Jolla, CA) and SPSS 25 for Mac (IBM Corp, Armonk, NY) software.