Anti β actin mouse monoclonal antibody a5316

The following antibodies were used: rabbit anti-human-GPR37-N polyclonal antibody [17 (link)], rabbit anti-mouse-GPR37-N polyclonal antibody [7 (link), 18 (link)], rabbit anti-pan-GPR37-C polyclonal antibody [18 (link)], mouse anti-β-actin monoclonal antibody (A5316; Sigma-Aldrich, St. Louis, MO) and rabbit anti-α-actinin polyclonal antibody (Santa Cruz Biotechnology Inc., Dallas, TX).

Sample collection and western blotting were performed in accordance with previously described methods [29 (link)]. Rabbit monoclonal anti-RIG-I antibody (#3743S; Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-IFN-α 11 antibody (bs-7023R; Bioss Antibodies, Woburn, MA, USA), rabbit polyclonal anti-IFN-β antibody (GTX37658; GeneTex, Irvine, CA, USA), Rabbit monoclonal anti-RIG-I antibody (#700366; Invitrogen, Carlsbad, CA, USA), rabbit polyclonal anti-IFN-α2 antibody (ab193055; Abcam, Cambridge, UK), rabbit polyclonal anti-IFN-β antibody (PA5-20390; Invitrogen), rat monoclonal anti-human klotho antibody (KO603; TransGenic, Fukuoka, Japan), mouse monoclonal anti-β-actin antibody (A5316; Sigma-Aldrich, St. Louis, MO, USA), and mouse monoclonal anti-α-tubulin antibody (T9026; Sigma-Aldrich) were used as primary antibodies. Horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Dako, Glostrup, Denmark) and goat anti-mouse immunoglobulin G (Dako) were used as secondary antibodies. SuperSignal West Dura and the Pico system (Thermo Fisher Scientific) were used to detect signals. The intensity of each band was measured by ImageJ software (version 1.47v; National Institutes of Health, Bethesda, MD, USA) and normalized to the level of either β-actin or α-tubulin.

Goat polyclonal anti-CXCR3 (sc-9901), rabbit polyclonal anti-LHR (sc-25828) and anti-WT1 (sc-192) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-Caspase-3 (9662S) and mouse monoclonal anti-Caspase-8 (9746S) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal anti-MuV nucleoprotein (ab9876) and rabbit polyclonal anti-MVH (ab13840) antibodies were purchased from Abcam (Cambridge, UK). Mouse monoclonal anti-β-actin antibody (A5316) was purchased from Sigma (St. Louis, MO, USA). Horseradish-peroxidase (HRP)-conjugated secondary antibodies were purchased from Zhongshan Biotechnology Co. (Beijing, China). Recombinant mouse CXCL10 (250-16) and TNF-α (315-01A) were purchased from Peprotech (Rocky Hill, CT, USA). DEVD-fmk (264156), an inhibitor of caspase-3, was purchased from Calbiochem (La Jolla, CA, USA). Pomalidomide (S1567), an inhibitors of TNF-α, was purchased from Selleckchem (Houston, TX, USA). Annexin V-FITC apoptosis detection kit (FXP018) and ELISA kit for detecting mouse TNF-α (CME0004) were purchased from Beijing 4A Biotech Company (Beijing, China). ELISA kit for detecting mouse CXCL10 (BMS6018) was purchased from eBioscience (San Diego, CA, USA).