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Dimethyl malonate

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Dimethyl malonate is a chemical compound used in various laboratory applications. It is a colorless liquid with a characteristic odor. Dimethyl malonate serves as a versatile building block for organic synthesis, particularly in the preparation of other chemical compounds.

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Lab products found in correlation

Polypropylene tube, by Sarstedt (2 mentions) 2 deoxyglucose 2dg, by Thermo Fisher Scientific (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Thermo Fisher Scientific (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Antimycin a, by Thermo Fisher Scientific (1 mentions) Hoescht 33342, by Thermo Fisher Scientific (1 mentions) Rotenone, by Thermo Fisher Scientific (1 mentions) Oligomycin, by Thermo Fisher Scientific (1 mentions) Etomoxir, by Thermo Fisher Scientific (1 mentions) Vas2870, by Cayman Chemical (1 mentions) Sotrastaurin, by Cayman Chemical (1 mentions) Atpenin a5, by Cayman Chemical (1 mentions) Pam3csk4, by InvivoGen (1 mentions)

3 protocols using dimethyl malonate

1

Metabolic Modulation in Immune Cells

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All reagents were from Sigma unless otherwise stated. Specific kits and linked reagents are described below. Non-canonical reagents are listed.
Rotenone, dehydroepiandrosterone (DHEA), thenoyltrifluoroacetone (TTFA), antimycin A, 2-deoxyglucose (2-DG), oligomycin, glutamate (pH 7.4 with NaOH), zymosan particles from Saccharomyces cerevisiae (including pHrodo, Thermo-Fisher), Yeast from Saccharomyces cerevisiae, LPS (lipopolysaccharide), luminol, DTAC (Dodecyltrimethylammonium chloride), Accutase, diazo-oxo-norleucine (DON), bis-phenylacetamido-thiadiazolyl-ethyl sulphide (BPTES), atpenin A5 (Cayman chemicals), dimethylmalonate, UK-5099, etomoxir, phorbol–myristate–acetate (PMA), mitotracker red CMX-Ros (Thermo-Fisher), all-trans retinoic acid, M-CSF (Peprotech), VAS-2870, Sotrastaurin (Cayman chemicals), N-Formylmethionyl-leucyl-phenylalanine (fMLF), Pam3CSK4 (Invivogen), uric acid crystals, R848 (Invivogen), 4′,6-Diamidino-2-Phenylindole (DAPI, Thermo-Fisher), Hoescht 33342 (Thermo-Fisher), 2-mercaptoethanol.
Davies L.C., Rice C.M., Palmieri E.M., Taylor P.R., Kuhns D.B, & McVicar D.W. (2017). Peritoneal tissue-resident macrophages are metabolically poised to engage microbes using tissue-niche fuels. Nature Communications, 8, 2074.
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2

Tissue Sample Incubation Protocols

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The composition of UW storage solution used is (mM): potassium lactobionate, 50; KH2PO4, 25; MgSO4, 5; raffinose, 30; adenosine, 5; glutathione, 3; allopurinol, 1; and hydroxyethyl starch (50 g/L). The composition of St Thomas’s cardioplegia solution is: (NaCl 110.0 mM, NaHCO3 10.0 mM, KCl 16.0 mM, MgCl2 16.0 mM, CaCl2 1.2 mM, pH 7.8). Dimethyl malonate was from Thermo Fisher Scientific, UK. Tissue samples were incubated in polypropylene tubes, either 1.5 ml (Ref:72.690.001) or 50 mL (Ref: 62.547.004), both from Sarstedt, Nümbrecht, Germany.
Martin J.L., Costa A.S., Gruszczyk A.V., Beach T.E., Allen F.M., Prag H.A., Hinchy E.C., Mahbubani K., Hamed M., Tronci L., Nikitopoulou E., James A.M., Krieg T., Robinson A.J., Huang M.H., Caldwell S.T., Logan A., Pala L., Hartley R.C., Frezza C., Saeb-Parsy K, & Murphy M.P. (2019). Succinate accumulation drives ischaemia-reperfusion injury during organ transplantation. Nature metabolism, 1, 966-974.
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3

Tissue Sample Incubation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
The composition of UW storage solution used is (mM): potassium lactobionate, 50; KH2PO4, 25; MgSO4, 5; raffinose, 30; adenosine, 5; glutathione, 3; allopurinol, 1; and hydroxyethyl starch (50 g/L). The composition of St Thomas’s cardioplegia solution is: (NaCl 110.0 mM, NaHCO3 10.0 mM, KCl 16.0 mM, MgCl2 16.0 mM, CaCl2 1.2 mM, pH 7.8). Dimethyl malonate was from Thermo Fisher Scientific, UK. Tissue samples were incubated in polypropylene tubes, either 1.5 ml (Ref:72.690.001) or 50 mL (Ref: 62.547.004), both from Sarstedt, Nümbrecht, Germany.
Martin J.L., Costa A.S., Gruszczyk A.V., Beach T.E., Allen F.M., Prag H.A., Hinchy E.C., Mahbubani K., Hamed M., Tronci L., Nikitopoulou E., James A.M., Krieg T., Robinson A.J., Huang M.H., Caldwell S.T., Logan A., Pala L., Hartley R.C., Frezza C., Saeb-Parsy K, & Murphy M.P. (2019). Succinate accumulation drives ischaemia-reperfusion injury during organ transplantation. Nature metabolism, 1, 966-974.
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