Glucose levels in blood samples obtained from tail vein were measured with an Accu-Check glucometer (Roche). Serum insulin level was determined with a Mouse Insulin ELISA Kit (Shibayagi). Serum ALT, AST, TG and cholesterol levels were measured with a Fuji Dri-Chem 3500 biochemistry analyzer (Fujifilm). Serum GDF15 level was determined using a Mouse GDF-15 DuoSet ELISA Kit (R&D Systems) or a Human GDF-15 Quantikine ELISA Kit. Serum FFA level was determined using a SICDIA NEFAZYME Kit (Shinyang Chemical). Liver TG content was measured using a Triglyceride Kit (GPO-Trinder, Sigma). HOMA-IR index was calculated according to the following formula: [fasting insulin (μlU/ml) × fasting glucose (mg/dl)]/405.
Fuji dri chem 3500 biochemistry analyzer
Manufactured by Fujifilm
Sourced in Japan
The Fuji Dri-Chem 3500 is a biochemistry analyzer designed for clinical laboratory testing. It utilizes dry chemical technology to perform a variety of biochemical assays on small sample volumes. The core function of the Fuji Dri-Chem 3500 is to analyze and provide quantitative results for various analytes in biological samples such as blood, serum, and urine.
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3 protocols using fuji dri chem 3500 biochemistry analyzer
1
Metabolic Profiling of Mice
2
Pharmacokinetics and Toxicity Evaluation of K202.B
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In vivo toxicity and serum pharmacokinetic studies using animals were approved by the IACUC (Approval No. NCC-21-693) of the National Cancer Center, Republic of Korea. Eight-week-old female Institute of Cancer Research (ICR) mice (Orient Bio Inc., Seongnam, Republic of Korea) were intravenously injected with 5 or 30 mg/kg of K202.B (n = 3 per group). At 4, 8, 24, 72, 120, 168, 264, 384, and 504 h post-inoculation, blood samples (50 μL) were collected from each mouse and centrifuged at 5000×g for 20 min at 4 °C. The serum was stored at −80 °C for evaluation of biochemical parameters. Serum levels of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), creatinine (CRE), and blood urea nitrogen (BUN) were measured using a Fuji Dri-Chem 3500 Biochemistry Analyzer (Fujifilm, Tokyo, Japan). Serum levels of K202.B were determined using a human IgG ELISA kit (Abcam) according to the manufacturer's instructions. Optical density was measured using a Synergy H1 microplate reader, and values were compared to those from a concurrently analyzed standard curve.
3
Evaluating GRP94 IgG Toxicity in Mice
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In vivo toxicity testing was performed as described previously [12 (link)]. Bagg albino (BALB)/c-nude mice (n = 4) were injected intravenously twice weekly with or without 10 mg/kg GRP94 IgG, and their body weights were measured weekly. After 42 days, mice were sacrificed and blood samples were collected. Serum glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), total bilirubin (TBIL), blood urea nitrogen (BUN), and creatinine (CRE) were measured using a Fuji Dri-Chem 3500 biochemistry analyzer (Fujifilm, Tokyo, Japan).
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