Human fetal lung tissue samples were acquired through the tissue retrieval program sponsored by the National Institute of Child Health and Development, the University of Maryland Brain and Tissue Bank for Developmental Disorders (Baltimore, MD, USA), and the Center for Birth Defects Research (University of Washington, Seattle, WA, USA) [13 (link)]. Gene expression profiles were generated using the Affymetrix Human 1.0 ST Array. All human and animal studies were approved by the Brigham and Women’s Hospital Institutional Review Board.
RNA was extracted from fetal tissue whole lung homogenate and RNA sample quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.,Santa Clara, CA, USA). Genome-wide gene expression profiles were generated using the Affymetrix Human 1.0 ST Array. The pre-processing of the gene expression data was performed including background correction and quantile normalization prior to analysis. The probe with the highest variance for each miRNA was used as the representation of each miRNA. In total, ~200 of the known miRNAs were interrogated on this Affymetrix platform.
RNA was extracted from fetal tissue whole lung homogenate and RNA sample quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.,Santa Clara, CA, USA). Genome-wide gene expression profiles were generated using the Affymetrix Human 1.0 ST Array. The pre-processing of the gene expression data was performed including background correction and quantile normalization prior to analysis. The probe with the highest variance for each miRNA was used as the representation of each miRNA. In total, ~200 of the known miRNAs were interrogated on this Affymetrix platform.