The following chemicals were purchased from Sigma Aldrich (Taufkirchen, Germany): 2,2′-azobis(2-methylpropionitrile) (AIBN), ethanolamine, deuterated chloroform, isobutyronitrile, methyl trifluoromethanesulfonate, N-methylpropargylamine, THF (HPLC grade), valeronitrile and zinc acetate dihydrate. N,N-diethylamine and propargyl tosylate were purchased from Fluka (Taufkirchen, Germany). Calcium hydride was bought from Alfa Aesar (Karlsruhe, Germany). Sodium chloride and sodium sulfate were bought from Roth (Karlsruhe, Germany). Ce(SO4)·4H2O and (NH4)6Mo7O24·4H2O were obtained from VEB (Eschborn, Germany). Concentrated sulfuric acid was purchased from Th. Geyer (Renningen, Germany). deuterated chloroform was obtained from Chemotrade (Düsseldorf, Germany), and DMF (HPLC grade) was purchased from VWR-Prolabo (Darmstadt, Germany).
Aβ40 peptide was synthesized using the standard F-moc solid phase synthesis strategy at the core unit “Peptid-Technologien” of the Medical Faculty of the University of Leipzig [52 (link)].
The oxazoline monomers were stored over Calcium hydride and were freshly distilled before usage. All solvents were freshly distilled and degassed by bubbling with nitrogen for at least 20 min prior to usage. Acetonitrile (ACN) was dried with Calcium hydride by boiling for several hours followed by distillation. AIBN was recrystallized from ethanol twice before usage.
Aβ40 peptide was synthesized using the standard F-moc solid phase synthesis strategy at the core unit “Peptid-Technologien” of the Medical Faculty of the University of Leipzig [52 (link)].
The oxazoline monomers were stored over Calcium hydride and were freshly distilled before usage. All solvents were freshly distilled and degassed by bubbling with nitrogen for at least 20 min prior to usage. Acetonitrile (ACN) was dried with Calcium hydride by boiling for several hours followed by distillation. AIBN was recrystallized from ethanol twice before usage.