Rabbit polyclonal anti-TRAF2 (#sc-876), mouse monoclonal β-actin (#sc-47778), mouse monoclonal anti-USP-14 (#sc-100630), mouse monoclonal anti-MMP-1 (#sc-58377), rabbit polyclonal anti-MMP-13 (#sc-30073), rabbit polyclonal anti-Lamin A/C (#sc-20681) and rabbit polyclonal anti-p-IkB-α (#sc-8404) antibodies were purchased from Santa Cruz Biotech, (Santa Cruz, CA). Rabbit monoclonal Anti-cIAP1 (#7065), rabbit monoclonal anti-cIAP2 (#3031), rabbit anti-USP2 (#8036), rabbit monoclonal anti-RAD23A (#24555), anti-K63 polyubiquitin (#5621), and anti-K48 polyubiquitin (#8081), anti-STAT-3 (#9132), anti-NF-κBp65 (#8242), anti-p-c-Jun (S73) (#9164), anti-p-p38 (T180/Y182) (#4511), anti-p-JNK (T183/Y185) (#9251), total JNK (#8690), total p-38 (# 9258) and p-STAT-3 (S727) (#9134) antibodies were purchased from Cell Signaling Technology (Beverly, MA). TRAF2 mouse monoclonal (#AM1895B) for immunoprecipitation were purchased from Abjent (San Diego, CA). Anti-PSMD13 (#5937-1) antibody was purchased from Epitomics (Burlingame, CA), respectively. Total ASK1 (#ab131506), p-ASK1 Thr838/845 antibodies were was purchased from Abcam (Cambridge, MA) and Cell Signaling Technology (Beverly, MA), respectively. Human Cytokine Array C5 (#AAH-CYT-5) was purchased from Ray Biotech (Norcross, GA). SMARTpool ON-TARGET plus ASK1 siRNA or negative control siRNA was purchased from GE Dharmacon (Lafayette, CO).
Rabbit polyclonal anti mmp 13
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rabbit polyclonal anti-MMP-13 is an antibody produced in rabbits that targets the Matrix Metalloproteinase-13 (MMP-13) protein. MMP-13 is an enzyme involved in the breakdown of extracellular matrix components.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal β actin, by Santa Cruz Biotechnology (1 mentions)
Anti nf κb p65, by Cell Signaling Technology (1 mentions)
Total p38, by Cell Signaling Technology (1 mentions)
Anti traf2, by Santa Cruz Biotechnology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Anti ciap1, by Cell Signaling Technology (1 mentions)
P stat3 s727, by Cell Signaling Technology (1 mentions)
Human cytokine array c5, by RayBiotech (1 mentions)
Anti k63 polyubiquitin, by Cell Signaling Technology (1 mentions)
Anti k48 polyubiquitin, by Cell Signaling Technology (1 mentions)
Anti p p38 t180 y182, by Cell Signaling Technology (1 mentions)
Anti p jnk t183 y185, by Cell Signaling Technology (1 mentions)
Polyclonal anti lamin a c, by Santa Cruz Biotechnology (1 mentions)
Rabbit monoclonal anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemiluminescent reagent, by Thermo Fisher Scientific (1 mentions)
Bradford assay, by Bio-Rad (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Mouse monoclonal anti actin, by Cell Signaling Technology (1 mentions)
Rabbit polyclonal anti gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit polyclonal anti mmp 13
1
Comprehensive Antibody Catalog for Protein Analysis
2
Protein Expression Analysis Using Western Blot
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Specimens were lysed in a buffer supplemented with a protease inhibitor cocktail (Roche) [14 (link)]. Concentrations of proteins were measured with the Bradford assay (Bio-Rad Laboratories, Richmond, CA, USA) using a spectrophotometer. Proteins (20 μg) were separated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Billerica, MA, USA). The following primary antibodies were incubated overnight with the blocked membranes: rabbit polyclonal anti-COL2A1, rabbit polyclonal anti-MMP-13 (Santa Cruz Biotechnology), goat polyclonal anti-BMAL1, goat polyclonal anti-CLOCK, rabbit polyclonal anti-OPG, goat polyclonal anti-PER1, goat polyclonal anti-PER2, goat polyclonal anti-RANKL, mouse monoclonal anti-actin, rabbit polyclonal anti-GAPDH, and rabbit monoclonal anti-cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA). Membranes were then incubated with the secondary antibody. Finally, specific bands were visualized with chemiluminescent reagents (Pierce Biotechnology, Rockford, IL, USA) and quantified using the ImageJ software.
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