Immunofluorescent staining of retinal frozen sections and cultured cells was performed as previously described (Xia et al., 2008 (link)). Antibodies used in the study: Ezrin rabbit polyclonal antibody (Cell Signaling Technology), NHE8 mouse monoclonal antibody (Chemicon International), TGN46 rabbit polyclonal antibody (Millipore), and HA mouse monoclonal antibody (BioLegend). All images were collected by a Zeiss LSM700 confocal microscope.
Tgn46 rabbit polyclonal antibody
Manufactured by Merck Group
The TGN46 rabbit polyclonal antibody is a laboratory reagent produced by Merck Group. It is designed for use in various immunological techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry. The antibody specifically recognizes the TGN46 protein, which is a marker for the trans-Golgi network in eukaryotic cells.
Automatically generated - may contain errors
2 protocols using tgn46 rabbit polyclonal antibody
1
Immunofluorescent Staining of Retinal Tissue
2
Immunofluorescent Staining of Retinal and RPE Samples
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Immunofluorescent staining of retinal frozen sections was performed as previously described41 (link). For staining RPE whole mount, mouse eyes were fixed in 4% formaldehyde/PBS, the cornea and lens were removed, followed by antibody staining and the remaining eye cups were flat-mounted for imaging. A rabbit polyclonal antibody for NHE8 was generated using the C-terminal 14-amino acid peptide as immunogen; other antibodies used for immunostaining were: RPE65 mouse monoclonal antibody (generously provided by Dr. Debra Thompson, University of Michigan), ezrin rabbit polyclonal antibody (Cell Signaling Technology), ZO-1 rabbit polyclonal antibody (Invitrogen Life Technologies), giantin mouse monoclonal antibody42 (link), TGN46 rabbit polyclonal antibody (Millipore), calreticulin rabbit polyclonal antibody (Stressgen Biotechnologies) and rhodamine phalloidin (Molecular Probes). All images were collected by a Zeiss LSM700 confocal microscope.
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