Rapid ana 2

The specimens of gingival exudate or plaque in the periodontal pocket, stomach mucosa and drainage fluid from the abdominal cavity were stored in an anaerobic culture tube II (TERUMO Corporation, Tokyo Japan). A smear test, aerobic culturing and anaerobic culturing were performed using TSA II with 5% sheep’s blood/chocolate II medium (Becton, Dickinson and Co., NJ, USA), Bromothymol Blue Lactose medium (Becton, Dickinson and Co., NJ, USA), Anaero Columbia RS blood medium (Becton, Dickinson and Co., NJ, USA) and CO₂ incubation. Pure culture and identification were performed the day after culturing. Aerobic bacteria were identified using a MicroScan WalkAway 96 Plus (Becton, Dickinson and Co., NJ, USA), and anaerobic bacteria were identified using a RapID ANA II (Thermo Fisher Scientific, MA, USA).

A total of 20 mL of whole blood samples (for one aerobic blood culture bottle and one anaerobic blood culture bottle, respectively) were collected simultaneously with 2 mL of whole blood sample for Tm mapping method from the same puncture site. The whole-blood samples were then analyzed according to standard methods used by the Clinical Laboratory Center (certified ISO15189) at Toyama University Hospital. The blood culture procedures were performed using the BacT/ALERT 3D system (bioMerieux, Inc., Mercy-l’Etoile, France). Positive blood culture bottles were subcultured onto sheep blood agar, chocolate agar, and BTB lactose-contained agar. Moreover, as a result of microscopic examination, added appropriate media if necessary. Then, they were incubated aerobically or anaerobically until sufficient growth was present to proceed with testing (usually 18–24 h). The specific identification methods differed according to the organism, although they involved the MicroScan WalkAway system (Siemens Healthcare Diagnostics, IL, USA), RapID ANA II (Thermo Fisher Scientific, UK), and various latex agglutination and biochemical spot tests.