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Mouse il 21 duoset elisa

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The Mouse IL-21 DuoSet ELISA is a laboratory assay kit used to quantitatively measure mouse interleukin-21 (IL-21) levels in cell culture supernatants, serum, and plasma samples. The kit includes the necessary components to perform a sandwich enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Elisa max deluxe set, by BioLegend (1 mentions) Opteia mouse il 10 elisa set, by BD (1 mentions) Opteia mouse ifn γ elisaset, by BD (1 mentions) Opteia human ifn γ elisa set, by BD (1 mentions) Human il 21 duoset elisa, by R&D Systems (1 mentions) Human il 9 duoset elisa, by R&D Systems (1 mentions)

2 protocols using mouse il 21 duoset elisa

1

Quantifying Th17 Cell Cytokines

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Mouse IL-17A and IFN-γ ELISA MAX Deluxe sets (BioLegend) and Mouse IL-21 DuoSet Elisa (R&D) were used to measure IL-17A, IFN-γ, and IL-21 from the culture supernatant of Th17 cultures from CD4+ T cells isolated from WT or Tec-deficient mice.
Sandner L., Alteneder M., Zhu C., Hladik A., Högler S., Rica R., Van Greuningen L.W., Sharif O., Sakaguchi S., Knapp S., Kenner L., Trauner M., Ellmeier W, & Boucheron N. (2021). The Tyrosine Kinase Tec Regulates Effector Th17 Differentiation, Pathogenicity, and Plasticity in T-Cell-Driven Intestinal Inflammation. Frontiers in Immunology, 12, 750466.
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2

Cytokine Profiling using ELISA Assays

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The enzyme-linked immunosorbent assay (ELISA) technique was utilized to analyze the cytokine concentration in cell culture supernatants, BALF and Serum. ELISA was performed in accordance with the manufacturer’s instructions. Human ELISA Sets were purchased as followed: IL-9 ELISA (Human IL-9 DuoSet ELISA, R&D Systems, Cat# DY209-05), IL-21 ELISA (Human IL-21 DuoSet ELISA, R&D Systems, Cat# DY8879-05), IFNγ ELISA (BD OptEIA™ Human IFN-γ ELISA Set, BD Biosciences, Cat# 555142). Murine samples were analyzed by: IL-21 ELISA (Mouse IL-21 DuoSet ELISA, R&D Systems, Cat# DY594), IFNγ ELISA (BD OptEIA™ Mouse IFNγ ELISA Set, BD Biosciences, Cat# 555138), IL-10 ELISA (BD OptEIA™ Mouse IL-10 ELISA Set, BD Biosciences, Cat# 555252).
Heim L., Yang Z., Tausche P., Hohenberger K., Chiriac M.T., Koelle J., Geppert C.I., Kachler K., Miksch S., Graser A., Friedrich J., Kharwadkar R., Rieker R.J., Trufa D.I., Sirbu H., Neurath M.F., Kaplan M.H, & Finotto S. (2022). IL-9 Producing Tumor-Infiltrating Lymphocytes and Treg Subsets Drive Immune Escape of Tumor Cells in Non-Small Cell Lung Cancer. Frontiers in Immunology, 13, 859738.
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