Nb100 62652

Rabbit polyclonal anti-HBc antibody is from DAKO (B0586). For the detection of capsids, mouse monoclonal anti-HBc C1 (ab8637) was purchased from Abcam, and mouse monoclonal anti-HBc 2A7 was kindly provided by Prof. Quan Yuan (Xiamen University, China). For the immunoprecipitation of HBV virions, rabbit anti-HBs (NB100-62652, Novus, Centennial, CO, USA) and rabbit anti-preS1 antibodies (kindly provided by Prof. Shuping Tong, Brown University, USA) were used. For immunofluorescence staining, commercially available rabbit polyclonal anti-Alix (12422-1-AP, Proteintech, Chicago, IL, USA), and rabbit polyclonal anti-HBc (0282, Long Island Antibody, Shanghai, China) were used. Other commercially available antibodies are as follows: mouse anti-β-tubulin (M20005, Abmart, Shanghai, China), mouse anti-β-actin (T40104, Abmart, Shanghai, China), and rabbit anti-Flag (14793S, Cell Signaling Technology, Boston, MA, USA).

HBV stocks were prepared from HepAD 38 cells as described [34 (link)]. HepG2-NTCP cells were incubated for 16 h at 37°C with viral inoculum (500 Genome equivalent/ml) that had been preincubated for 10 min at room temperature with 0.001~1 μg/ml of Lenvervimab or polyclonal anti-HBsAg rabbit antibody (NB100-62652, Novus Biologicals, Centennial, CO, USA) in DMEM containing 10% FBS, 4% PEG 8000 and 2.5% DMSO. Viral inoculum was removed after 16 h and replaced with fresh medium containing 2.5% DMSO but no PEG 8000. HBsAg in the culture media was measured after 5 days by ELISA (Genedia HBsAg ELISA 3.0, Korea Green Cross, Yongin, Korea), and intracellular core proteins were analyzed by immunoblotting with rabbit anti-HBcAg antibody as described [35 (link)].