Sigma nad nadh quantitation kit

Manufactured by Merck Group

The SIGMA NAD/NADH Quantitation kit is a laboratory tool designed to quantify levels of nicotinamide adenine dinucleotide (NAD) and its reduced form, NADH, in biological samples. The kit provides a colorimetric method for the detection and measurement of these important cofactors.

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NAD/NADH Quantitation Kit NAD+/NADH kit Sigma kits

2 protocols using sigma nad nadh quantitation kit

Quantitative Analysis of NAD+

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Quantification of NAD+ was conducted with SIGMA NAD/NADH Quantitation kit (MAK037) following the manufacturer instructions. Briefly, 1 × 10⁵ cells were harvested for total NAD extraction. NAD+ and NADH were recognized by the NAD Cycling Enzyme Mix. The absorbance at 450 nm (A450) was measured. The NAD+ concentration was obtained by subtracting NADH from NADtotal.

Lai X., Huang S., Lin S., Pu L., Wang Y., Lin Y., Huang W, & Wang Z. (2022). Mesenchymal stromal cells attenuate alveolar type 2 cells senescence through regulating NAMPT-mediated NAD metabolism. Stem Cell Research & Therapy, 13, 12.

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Quantification of Intracellular NAD+

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Measurement of NAD⁺ was conducted using SIGMA NAD/NADH Quantitation kit (MAK037) following the manufacturer protocol. Briefly, 1 × 10⁵ cells harvested for total NAD extraction and quantification. The NAD Cycling Enzyme Mix recognizes NAD⁺ and NADH instead of NADP or NADPH. Concentration of NAD total (NAD⁺ + NADH) was measured by absorbance at OD 450 nm. The NAD⁺ concentration was calculated by subtracting the NADH values from NADtotal.

Ye C., Qi L., Li X., Wang J., Yu J., Zhou B., Guo C., Chen J, & Zheng S. (2020). Targeting the NAD+ salvage pathway suppresses APC mutation-driven colorectal cancer growth and Wnt/β-catenin signaling via increasing Axin level. Cell Communication and Signaling : CCS, 18, 16.

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