Cd45.2 percp

The osteosarcoma cell lines 143B, G-63, Saos-2, U2OS, SJSA-1 and HOS, and the Vero cells were obtained from the American Type Culture Collection (ATCC). RPMI-1640 medium and Dulbecco's modi ed Eagle's medium (DMEM)were purchased form Hyclone ( Logan, USA). Fetal bovine serum and L-glutamine were purchased form Gibco (Gaithersburg, USA). Penicillin and streptomycin were obtained from Sangong Biotech (Shanghai, China). HY-50847 were obtained from MCE (NJ, USA). Antibodies against IRE1α, p-ERK and ATF6 were purchased form Santa Cruz Biotechnology (TX, USA). Antibodies against CHOP and Ki-67 were purchased form Cell Signaling Technology (MA, USA). Antibody against caspase-12 were purchased form Abcam (OR, USA). Antibody against GAPDH were purchased form Bioworld (MN, USA). CD90.2-PerCP, CD45.2-perCP, CD8a-FITC, CD_4-PE, Gr1-APC, CD11b-PE, F4/80-FITC and NK1.1-PE were purchased form BioLegend (CA, USA).