Lipofectamine lipo 2000

CRC SW480 cells were purchased from KeyGen Biotech Co., Ltd. (Nanjing, China); Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Biological Industries (Beit-Haemek, Israel); fetal bovine serum (FBS) was purchased from SeraPro (Germany); quantitative reverse transcription polymerase chain reaction (qRT-PCR) SuperMix was purchased from TransGen Biotech Co., Ltd. (Beijing, China); TRIzol and Lipofectamine (Lipo) 2000 were purchased from Invitrogen (Waltham, MA, USA); miR-NC and miR-204 mimic were purchased from RiboBio Co., Ltd. (Guangzhou, China); EdU cell proliferation flow assay kit was purchased from Sigma-Aldrich (St Louis, MO, USA); rabbit anti-ANGPTL2 antibody was obtained from Abacm (Cambridge, MA, USA); rabbit anti-β-actin antibody was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA); HRP-conjugated secondary antibody was purchased from Sangon Biotechnology (Shanghai, China); pMIR plasmid was purchased from BioVector NTCC Inc. (Beijing, China); Dual-Glo Luciferase Assay System was purchased from Promega Corporation (Madison, WI, USA); radio-immune precipitation assay (RIPA) lysate was purchased from Hangzhou Multi Science (Lianke) Biotech Co. (Hangzhou, China); BeyoECL Plus chemiluminescence solution and Annexin V-FITC Apoptosis Detection Kit with propidium iodide (PI) were purchased from Suo Labao Bio (Beijing, China).