Azd8055

Manufactured by Active Motif

AZD8055 is a potent and selective inhibitor of the serine/threonine protein kinase mTOR (mammalian target of rapamycin). It acts by directly binding to the catalytic domain of mTOR, thereby inhibiting its kinase activity.

Automatically generated - may contain errors

Lab products found in correlation

Polyclonal and monoclonal actin antibodies, by Merck Group (1 mentions) Torin1, by Bio-Techne (1 mentions) Sb216763, by Merck Group (1 mentions) Pp242, by Active Motif (1 mentions) Ink128, by Active Motif (1 mentions) Mg132, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) Phospho fak y397, by Abcam (1 mentions) Atg13, by Cell Signaling Technology (1 mentions) Phospho beclin 1 s15, by Abbiotec (1 mentions) Glucose free media, by Thermo Fisher Scientific (1 mentions) Pe annexin 5 apoptosis detection kit, by BD (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions)

3 protocols using azd8055

Investigating Signaling Pathways Regulating Cyclin D1

Check if the same lab product or an alternative is used in the 5 most similar protocols

PP242, INK128 and AZD8055 were purchased from Active Biochem (Maplewood, NJ). Torin 1 was purchased from Tocris (Bristol, UK). The GSK3 inhibitor SB216763, the proteasome inhibitor MG132 and the protein synthesis inhibitor cycloheximide (CHX) were purchased from Sigma Chemical Co. (St. Louis, MO). The NEDD8-activating enzyme inhibitor MLN4924 was provided by Millennium Pharmaceuticals, Inc (Cambridge, MA). Cyclin D1, p-GSK3α/β (S21/9), p-AKT (S473), AKT, p-S6 (S235/236), and S6 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). GSK3α/β antibody was purchased from Upstate/EMD Millipore (Billerica, MA). Polyclonal rictor and raptor antibodies were purchased from Bethyl Laboratories, Inc. (Montgomery, TX). Both polyclonal and monoclonal actin antibodies were purchased from Sigma Chemical Co. Myc-tagged constitutively active form of GSK3β (GSK3βCA) (24 (link)) was provided by Dr. Binhua P. Zhou (The University of Kentucky, College of Medicine, Lexington, Kentucky). Flag-cyclin D1 expression plasmid was provided by Dr. Alan Diehl (Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA). Myc-Rictor and HA-raptor expression plasmids were purchased from Addegene (Cambridge MA).

Koo J., Yue P., Gal A.A., Khuri F.R, & Sun S.Y. (2014). Maintaining glycogen synthase kinase-3 activity is critical for mTOR kinase inhibitors to inhibit cancer cell growth. Cancer research, 74(9), 2555-2568.

+ Open protocol

+ Expand

Inhibitors of mTOR Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

MLN0128 was provided by Intellikine or purchased from Active Biochem (Maplewood, NJ). AZD8055 was purchased from Active Biochem, PP242 and Rapamycin from LC labs (Woburn, MA). All compounds were dissolved in DMSO at 10 mM and stored frozen in aliquots.

Mallya S., Fitch B.A., Lee J.S., So L., Janes M.R, & Fruman D.A. (2014). Resistance to mTOR Kinase Inhibitors in Lymphoma Cells Lacking 4EBP1. PLoS ONE, 9(2), e88865.

+ Open protocol

+ Expand

Autophagy Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell Signaling Antibodies used: Atg13 (#6940), Beclin1 (#3495), PARP (#9542), 4E-BP1 (#9452), phospho-ULK1 S757 (#6888), Ulk1 (#8054), p70 S6K (#9202), phospho-S6 S235/236 (#4858), Myc tag (#2278), LC3B (#3868), Vps34 (#4263), phospho-AMPK T172 (#2535), phospho-ACC S79 (#3661), phospho-Paxillin Y118 (#2541), and PathScan Multiplex Western Cocktail I (#5301). Phospho-Vps34 Ser249 antibody was developed in collaboration with Gary Kasof at Cell Signaling Technology. Sigma antibodies used: ULK1 (A7481), β-actin (A5541), and Flag tag polyclonal (F7425). Gabarap antibody from Abgent (PM037). Phospho-FAK Y397 from Abcam (ab4803). FAK from Epitomics (2146-1). p62 SQSTM1 antibody from Progen (03-GPP62-C). Phospho-Beclin1 S15 from Abbiotec (254515). Phospho-Atg13 S318 (human S355) from Rockland (600-401-C49S). EBSS (14155-063) and glucose-free media (11966-025) from Gibco/Life Technologies. Chloroquine (C6628) and bafilomycin A1 (B1793) from Sigma. AZD-8055 (A-1008) from Active Biochem. AnnexinV-PE Apoptosis Detection Kit from BD Biosciences. Phos-tag™ AAL-107 from NARD (#304-93521). Ad5-CMV-Cre was purchased from the University of Iowa viral vector core.

Egan D.F., Chun M.G., Vamos M., Zou H., Rong J., Miller C.J., Lou H.J., Raveendra-Panickar D., Yang C.C., Sheffler D.J., Teriete P., Asara J.M., Turk B.E., Cosford N.D, & Shaw R.J. (2015). Small molecule inhibition of the autophagy kinase ULK1 and identification of ULK1 substrates. Molecular cell, 59(2), 285-297.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!