Goat anti mouse igg fitc sc 2010

Beta nicotinamide mononucleotide (β-NMN) (BTO5) was purchased from BONTAC (Shenzhen, China). Nicotinamide phosphoribosyltransferase (Nampt) inhibitor (GMX1778) was from Selleck (Shanghai, China). Lomotil (Atropine-diphenoxylate) was purchased from Kangpu Pharmaceutical Ltd Co (Changzhou, China). Antibodies against serotonin transporter (ab181034), tryptophan hydroxylase (ab52954), Lgr5 (ab75850) and Nampt (ab45890) were purchased from Abcam (Cambridge, MA, USA). SR4 (sc-376158), p15/16 (sc-377412) and goat anti-mouse IgG-FITC (sc-2010) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). APC anti-mouse CD117 (c-kit) (135108) was purchased from BioLegend (San Diego, CA, USA). Proliferating cell nuclear antigen (2586), GAPDH (5174) and Senescence β-Galactosidase Staining Kit (9860) were purchased from Cell Signaling Technology (Danvers, MA, USA). Goat anti-mouse IgG (31430), goat anti-rabbit IgG (31460) and TRIzol Reagent (15596-018) were purchased from Invitrogen (Carlsbad, CA, USA). EvaGreen Supermix (172-5201AP) was purchased from Bio-Rad Laboratories Inc (Hercules, CA, USA). PrimeScript first strand cDNA Synthesis Kit (6110A) was purchased from TaKaRa Bio Inc (Dalian, China). Nuclear Fast Red solution (N3020) and all other chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA), unless otherwise stated.

To better visualize the cellular distribution and the localization of the HR-HPV16 E7 oncoprotein, the tumor tissue specimens were processed for fluorescence-based immunodetection. The sections immunoreacted with mouse monoclonal anti-HPV16 E7 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA, 1:50 dilution, sc-6981) overnight at 4 °C were washed in PBS and incubated with goat anti-mouse IgG-FITC: sc-2010 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA 1:300) as the secondary antibody. Then, sections were counterstained with 4′,6-diamidino-2phenylindole (DAPI) (Thermo Fisher Scientific, Invitrogen, Renfrew, UK, 1:3000) and mounted in Prolong Gold with DAPI (Thermo Fisher Scientific, Waltham, MA, USA). Imaging was performed using an Eclipse Ti-E confocal microscope (Nikon, Tokyo, Japan).
The workflow of the present study is summarized in Appendix A.

Poly(ethylene glycol) diacrylate (PEG-DA, MW 575), 2-hydroxy-2-methylpropiophenone (HOMPP), polycaprolactone (PCL, MW 80000), dimethylsulfoxide (DMSO), ethanol, Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), horse serum (HS) were purchased from Invitrogen (Carlsbad, CA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide (MTT) antibiotic/antimycotic solution, and trypsin/ethylenediaminetetra-acetate (trypsin/EDTA) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). C2C12 mouse myoblasts were purchased from the Korean Cell Line Bank (Seoul, Korea). Phosphate buffered saline (PBS, 0.1 M, pH 7.4) was purchased from Invitrogen (Carlsbad, CA, USA). Mouse monoclonal antibody to MHC (sc-376157) and goat anti-mouse IgG-FITC (sc-2010) were purchased from Santa Cruz (Dallas, TX, USA). Photomasks for photolithography were prepared using AutoCAD and were printed on transparencies using a standard laser jet printer (LaserWriter 16/600 PS, Apple, Inc., Cupertino, CA, USA).