Anti muc1 c

Manufactured by Lab Vision

Anti-MUC1-C is a monoclonal antibody that binds to the MUC1-C protein. MUC1-C is a transmembrane glycoprotein that is overexpressed in various types of cancer cells. The core function of Anti-MUC1-C is to detect and identify the presence of MUC1-C in biological samples.

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Lab products found in correlation

Anti β actin, by Merck Group (3 mentions) Enhanced chemiluminescence, by GE Healthcare (2 mentions) Anti nf κb p65, by Santa Cruz Biotechnology (2 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Anti cdk4, by Cell Signaling Technology (1 mentions) Anti rb, by BD (1 mentions) Anti myc, by Abcam (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by GE Healthcare (1 mentions) Anti lin28b, by Cell Signaling Technology (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions) Anti hmga2, by Cell Signaling Technology (1 mentions) Anti nf κb p65, by Cell Signaling Technology (1 mentions)

3 protocols using anti muc1 c

Immunoblot Analysis of Cell Signaling Proteins

Cited 1 time

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Whole cell lysates were prepared in NP-40 lysis buffer and analyzed by immunoblotting with anti-MUC1-C (LabVision), anti-MYC (Abcam), anti-β-actin (Sigma), anti-CDK4 (Cell Signaling Technology), anti-cyclin D1 (NeoMarkers), anti-phospho-Rb, and anti-Rb (BD Biosciences) as described (28 (link)). Immune complexes were detected with horseradish peroxidase secondary antibodies and enhanced chemiluminescence (GE Healthcare).

Bouillez A., Rajabi H., Pitroda S., Jin C., Alam M., Kharbanda A., Tagde A., Wong K.K, & Kufe D. (2016). INHIBITION OF MUC1-C SUPPRESSES MYC EXPRESSION AND ATTENUATES MALIGNANT GROWTH IN KRAS MUTANT LUNG ADENOCARCINOMAS. Cancer research, 76(6), 1538-1548.

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Immunoprecipitation and Immunoblotting Analysis

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Cells were lysed using NP-40 lysis buffer containing protease inhibitor cocktail (Thermo Scientific). Soluble proteins were immunoprecipitated with anti-NF-κB p65 (Santa Cruz Biotechnology) or a control IgG. Precipitates and lysates not subjected to precipitation were analyzed by immunoblotting with anti-MUC1-C (LabVision), anti-NF-κB p65, anti-LIN28B, anti-E-cadherin, anti-vimentin, anti-HMGA2 (Cell Signaling) and anti-β-actin (Sigma). Immunoreactive complexes were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) and an enhanced chemiluminescence (ECL) detection system (Perkin Elmer Health Sciences).

Alam M., Ahmad R., Rajabi H, & Kufe D. (2014). MUC1-C INDUCES THE LIN28B→LET-7→HMGA2 AXIS TO REGULATE SELF-RENEWAL IN NSCLC. Molecular cancer research : MCR, 13(3), 449-460.

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Protein Expression Analysis by Immunoblotting

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Whole cell lysates were prepared in NP-40 lysis buffer and analyzed by immunoblotting with anti-MUC1-C (LabVision), anti-NF-κB p65 (Santa Cruz Biotechnology) and anti-β-actin (Sigma) as described [25 ]. Immune complexes were detected using horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence (GE Healthcare).

Alam M., Rajabi H., Ahmad R., Jin C, & Kufe D. (2014). Targeting the MUC1-C oncoprotein inhibits self-renewal capacity of breast cancer cells. Oncotarget, 5(9), 2622-2634.

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