Anti ova ige

Manufactured by Southern Biotech

Sourced in United States

The Anti-OVA IgE is a laboratory product used for the detection and quantification of IgE antibodies specific to ovalbumin (OVA), a major allergen found in egg white. This assay provides a tool for researchers to study IgE-mediated immune responses to ovalbumin.

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Lab products found in correlation

Rat anti mouse ige, by Southern Biotech (2 mentions) Extravidin, by Merck Group (2 mentions)

Spelling variants of this product

Anti-IgE (5 citations)

2 protocols using anti ova ige

Anti-OVA IgE ELISA Protocol

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Anti-OVA IgE ELISA was performed using plates coated with rat anti-mouse IgE (Southern Biotechnology Associates, Birmingham, USA), serum, and biotinylated OVA, as previously described.²² (link) The reaction was developed with the streptavidin-peroxidase conjugate (Extravidin; Sigma), plus o-phenylene-diamine (OPD) and H₂O₂. The plate was read at 492 nm and the results were reported in arbitrary units (AU) using a positive reference serum as being 1000 units.

Antunes M.M., Coelho B.S., Vichi T.M., Santos E.A., Gondim F.K., Diniz A.B., Aguilar E.C., Cara D.C., Porto L.C., Castro I.C., Alvarez Leite J.I, & Teixeira L.G. (2019). Oral supplementation with capsaicin reduces oxidative stress and IL-33 on a food allergy murine model. The World Allergy Organization Journal, 12(7), 100045.

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Anti-OVA IgE ELISA Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-OVA IgE ELISA was performed using plates coated with rat anti-mouse IgE (Southern Biotechnology Associates, Birmingham, USA), serum, and biotinylated OVA, as previously described [14 (link)]. The reaction was developed with the streptavidin-peroxidase conjugate (ExtrAvidin; Sigma), plus o-phenylene-diamine (OPD) and H₂O₂. The plate was read at 492 nm and the results were reported in arbitrary units (AU) using a positive reference serum as being 1000 units.

Batista N.V., Fonseca R.C., Perez D., Pereira R.V., de Lima Alves J., Pinho V., Faria A.M, & Cara D.C. (2016). Lack of Platelet-Activating Factor Receptor Attenuates Experimental Food Allergy but Not Its Metabolic Alterations regarding Adipokine Levels. BioMed Research International, 2016, 8601359.

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