Anti glut9

Cell culture medium and human primary renal PTECs were provided by ScienCell (San Diego, CA, United States). Anti-SLC2A9 (URAT1) antibodies, TSG101, CD63, caspase-1, GSDMD, IL-1β, caspase-3, caspase-8, caspase-9, cytochrome c, Bcl-2, and Bax were purchased from ABclonal (Wuhan, China). Oxonic acid (OA) and UA were provided by Sigma (St. Louis, MO, United States). CIAS1/NALP3, GAPDH, and anti-GLUT9 were provided by Abcam (Cambridge, United Kingdom). In addition, the CCK-8 assay kit was provided by Jiwei Biological Technology (Shanghai, China).

LBPs (≥90%) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Potassium oxonate (97%) and allopurinol (≥99%) was purchased from Sigma-Aldrich Co. LLC (Shanghai, China). Uric acid (UA), serum creatinine (S_CR), blood urine nitrogen (BUN) and xanthine oxidase activity assay kit were purchased from Abcam Inc. (Cambridge, UK). The RNA isolation kit was obtained from Promega Biotechnology Co., Ltd. (Beijing, China). PrimeScript^TM RT reagent kit with gDNA Eraser and TB Green^® Premix Ex Taq™ II (Tli RNaseH Plus) was purchased from Takara Biomedical Technology Co., Ltd. (Dalian, China). Protease and phosphatase inhibitors were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Anti-OAT1, anti-OAT3 and β-actin antibody were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-GLUT9 was purchased from Abcam Inc. (Cambridge, UK). The Amersham ECL System was purchased from GE Healthcare (Pittsburgh, PA, USA). Other biochemical reagents and chemicals were of analytical grade.