Autostainer plus instrument

Manufactured by Agilent Technologies

Sourced in United States

The Autostainer Plus instrument is a fully automated slide staining system designed for immunohistochemistry and in situ hybridization applications. It provides consistent, high-quality staining results with minimum user intervention.

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Lab products found in correlation

Mta 1, by Beecher Instruments (1 mentions) Yap1 antibody, by Cell Signaling Technology (1 mentions) Pt link module, by Agilent Technologies (1 mentions) Envision system hrp, by Agilent Technologies (1 mentions) Dako envision flex high ph detection kit, by Agilent Technologies (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Nis elements imaging software, by Nikon (1 mentions)

Close spelling variants of this product

Autostainer instrument Autostainer Plus Autostainer

3 protocols using autostainer plus instrument

Immunohistochemical Profiling of Breast Tumors

Cited 2 times

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Tumours from the screening and randomised cohorts were assembled in tissue microarrays using a manual tissue arrayer (MTA-1; BeecherInstruments, Inc., Sun Prairie, WI). The pre-treatment process of deparaffinization, rehydration and epitope retrieval of the 4 μm sections was carried out using the PT Link module (Dako, Glostrup, Denmark). Staining procedure with YAP1 antibody (1:25, Cell Signaling Technology Inc., Danvers, MA, cat#4912) was performed using the Autostainer Plus instrument with the Envision Flex programme (Dako). The epitope used for raising the YAP1 antibody includes amino acid 100 (personal communication, Cell Signaling Technology Europe B.V.) and should therefore detect all to date known isoforms of YAP1 [3 (link)]. YAP1 was scored as overall intensity as either absent, weak, intermediate or strong by a research associate (SL) and a pathologist (GL). Expression of ER, Ki-67, cyclin D1 and amplification of CCND1 (randomised cohort) had been scored previously in both the randomised and screening cohorts [35 (link),45 (link),46 (link)].

Lehn S., Tobin N.P., Sims A.H., Stål O., Jirström K., Axelson H, & Landberg G. (2014). Decreased expression of Yes-associated protein is associated with outcome in the luminal A breast cancer subgroup and with an impaired tamoxifen response. BMC Cancer, 14, 119.

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Immunohistochemical Analysis of Tumor-Infiltrating Lymphocytes

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Bladders were fixed in 10% formalin and embedded in paraffin. Four micron thick sections were used for IHC staining for CD8 (1:1000, #MA5-13263, Invitrogen) and Foxp3 (1:400, #12653, Cell Signaling). IHC was performed using a Dako Autostainer Plus instrument (Dako, CO, USA), and anti-rabbit Dako EnVision+System-HRP (Dako). For each tumor, the HRP positive cells were counted per low-power (10X objective) field. Each tumor was evaluated with at least six fields in two separate sections.

Podojil J.R., Glaser A.P., Baker D., Courtois E.T., Fantini D., Yu Y., Eaton V., Sivajothi S., Chiang M., Das A., McLaughlin K.A., Robson P., Miller S.D, & Meeks J.J. (2020). Antibody targeting of B7-H4 enhances the immune response in urothelial carcinoma. Oncoimmunology, 9(1), 1744897.

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Immunohistochemical Analysis of Brain Tumor Markers

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TfR1, H-ferritin, L-ferritin, and RRM2 expression in normal brain and glioblastoma, and TfR1 expression in human brain microvascular endothelial cells were examined by immunohistochemical (IHC) staining of tissue samples from surgically resected glioblastoma tumors from patients or from animal experiments. Dead cells in tumor xenografts in GaM-treated animals were identified according to typical nuclear morphological changes: pyknosis (nuclear condensation), karyorhexis (nuclear fragmentation), and karyolysis (complete dissolution of the nuclear fragments). Percent dead cells were calculated out of a total of 1000 cells (viable and dead) counted. Human tissue was obtained from the Brain and Spinal Cord Tissue Bank of the Medical College of Wisconsin. IHC staining with specific primary antibodies was performed on a Dako Autostainer Plus Instrument using the Dako EnVision™ FLEX High pH Detection Kit protocol. Stained slides were visualized using a Nikon Eclipse 80i microscope equipped with a MicroPublisher 3.3 RTV color video camera (Q Imaging, Surrey, BC, Canada). The images were captured using NIS elements imaging software (Version 7.0, Nikon Instruments, Inc.).

Chitambar C.R., Al-Gizawiy M.M., Alhajala H.S., Pechman K.R., Wereley J.P., Wujek R., Clark P.A., Kuo J.S., Antholine W.E, & Schmainda K.M. (2018). Gallium Maltolate Disrupts Tumor Iron Metabolism and Retards the Growth of Glioblastoma by Inhibiting Mitochondrial Function and Ribonucleotide Reductase. Molecular cancer therapeutics, 17(6), 1240-1250.

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