The largest database of trusted experimental protocols

2 protocols using cd38 antibody

1

Flow Cytometry and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD34 antibody (#555821) and CD38 antibody (#555460) for flow cytometry conjugated with biotin or specific dyes as required were purchased from BD Biosciences; γ-H2AX antibody was purchased from BioLegend (#613402). Western blot-related antibodies were purchased from Cell Signaling Technologies [GAPDH (#2118); BRCA1 (#9010); BRCA2 (#10741); DNA Damage Antibody Sampler Kit, (#9947); H2AX (#2595); CHK2 (#2662); ATM (#2873); ATR (#2790); Bcl2 (#15071) BIM (#2819); STAT3 (#9139); p-STAT3 (#9145)].
+ Open protocol
+ Expand
2

Immunoprecipitation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein A/G beads used for immunoprecipitation, rabbit anti-c-Cbl, rabbit anti-Vav1, and p-Tyr antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). PureProteome Protein G Magnetic Beads were from Millipore (Billerica, MA). Antibodies for GAPDH, beta-actin, p-Erk1/2, ERK1/2 (rabbit), pan-SFK416, Lyn, Fgr, Lck, Fyn, Vav1, SLP-76, pY-p55/p85 PI3K, total p85 PI3K, HRP anti-mouse, and HRP anti-rabbit were from Cell Signaling (Danvers, MA). CD38 antibody was purchased from BD Pharmingen (San Jose, CA). M-PER Mammalian Protein Extraction Reagent lysis buffer was from Pierce (Rockford, IL). Propidium iodide, protease and phosphatase inhibitors, and DMSO were purchased from Sigma (St. Louis, MO).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!