Plko 1 shrna vectors

Manufactured by Horizon Discovery

The PLKO.1 shRNA vectors are a set of plasmid vectors designed for the expression of short hairpin RNA (shRNA) in mammalian cells. These vectors utilize the RNA polymerase III U6 promoter to drive the expression of shRNA, which can be used to knockdown target gene expression through RNA interference (RNAi) mechanisms.

Automatically generated - may contain errors

Lab products found in correlation

Endofree plasmid kit, by Qiagen (2 mentions) Sc 108080, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

PLKO.1 (10 citations) PLKO.1 vector (4 citations) ShRNA vectors (2 citations)

2 protocols using plko 1 shrna vectors

Targeted shRNA Knockdown for Ciliopathy

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A library of shRNAs specific to the 30 human ciliopathy genes was assembled using the resources available at the UNC Gene Therapy Center. Lists of shRNA constructs and sources are provided in the Supplementary Information (Supplementary Table 4). Plasmids used for in utero electroporation were prepared using the EndoFree Plasmid kit (Qiagen). For each gene, two pools of shRNAs containing two to three individual shRNAs were randomly combined and used for electroporation. Non-silencing scrambled shRNA (sc-108080, Santa Cruz), GIPZ or pLKO.1 shRNA vectors (GE Dharmacon and UNC Gene Therapy Center) were used as control. KE of shRNAs (fold change compared with control) was measured using quantitative real-time PCR and are indicated for each shRNA group. KE of 70% was used as a threshold for shRNA pools. Each pool consisted of an equivalent amount of different shRNAs (2 or 3 per pool) listed. For BBS8, only the effective pool of ShRNA containing three different shRNAs was used. shRNAs from Santa Cruz are prepooled (four shRNAs per gene) by the vendor.

Guo J., Higginbotham H., Li J., Nichols J., Hirt J., Ghukasyan V, & Anton E.S. (2015). Developmental disruptions underlying brain abnormalities in ciliopathies. Nature Communications, 6, 7857.

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Ciliopathy Genes shRNA Library

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A library of shRNAs specific to the 30 human ciliopathy genes was assembled using the resources available at the UNC Gene Therapy Center. Lists of shRNA constructs and sources are provided in the supplemental information (Supp. Table 4). Plasmids used for in utero electroporation were prepared using the EndoFree Plasmid kit (Qiagen). For each gene, two pools of shRNAs containing two to three individual shRNAs were randomly combined and used for electroporation. Non-silencing scrambled shRNA (sc-108080, Santa Cruz), GIPZ or pLKO.1 shRNA vectors (GE Dharmacon and UNC Gene Therapy Center) were used as control. Knockdown efficiency (KE) of shRNAs (fold change compared to control) was measured using quantitative real time-PCR and are indicated for each shRNA group. Knockdown efficiency of 70% was used as a threshold for shRNA pools. Each pool consisted of equivalent amount of different shRNAs (2 or 3/ pool) listed. For BBS8, only the effective pool of ShRNA containing three different shRNAs was used. shRNAs from Santa Cruz are prepooled (4shRNAs/gene) by the vendor.

Guo J., Higginbotham H., Li J., Nichols J., Hirt J., Ghukasyan V, & Anton E.S. (2015). Developmental disruptions underlying brain abnormalities in ciliopathies. Nature Communications, 6, 7857.

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