Nexera uhplc system

Polyphenol compounds of MAJ were quantitatively analyzed using a liquid chromatography mass spectrometer system, LC-MS-8040™ (Shimadzu, Kyoto, Japan) with an electrospray ionization probe and a Nexera UHPLC system equipped with a phenyl-hexyl column (2.1 × 100 mm, 3.5 μm; Agilent, Santa Clara, CA, USA). The mobile phase was composed of solvents A (0.1% formic acid) and B (0.1% formic acid in acetonitrile), and the gradient was 95:5 at 1.5 min to 65:35 at 8 min to 0:100 at 10 min, followed by washing and reconditioning of the column. The flow rate was 0.4 mL/min, and the eluent was detected at 280 nm. Standard polyphenol compounds purchased from Sigma-Aldrich Co. were identified as previously reported [38 (link), 39 (link)].

Polyphenols of RCSF1 were quantitatively analyzed using a LCMS-8040™ liquid chromatography mass spectrometer (Shimadzu, Kyoto, Japan) with an electrospray ionization probe and a Nexera UHPLC system equipped with a phenyl-hexyl column (2.1 × 100 mm, 3.5 μm; Agilent, Santa Clara, CA, USA). Detailed multiple reaction monitoring conditions of target analytes are indicated in Table S1. Standard polyphenols purchased from Sigma-Aldrich were identified as previously reported [11 (link)].