Log In Sign up
The largest database of trusted experimental protocols

96 well black tissue culture plates

Manufactured by Corning
Visit Supplier
Sourced in United States

The 96-well black tissue culture plates are a standard laboratory equipment used for various cell-based assays and experiments. These plates provide a consistent and controlled environment for culturing cells, allowing for efficient and reproducible results. The black color of the plates helps to minimize background fluorescence, making them well-suited for applications that involve fluorescent or luminescent readouts.

Automatically generated - may contain errors

Lab products found in correlation

Celltiter glo luminescent cell viability assay kit, by Promega (2 mentions) Microplate reader, by Agilent Technologies (2 mentions) Metamorph software, by Universal Imaging (1 mentions)

Spelling variants of this product

96-well plates (3030 citations) 96-well culture plates (171 citations) Black 96-well plate (168 citations) 96-well tissue culture plates (144 citations) Tissue culture plates (66 citations) 96 wells (10 citations) 96-well black culture plate (3 citations) 96-well clear bottom black tissue culture treated plates (2 citations)

5 protocols using 96 well black tissue culture plates

1

SARS-CoV Antiviral Replication and Cytotoxicity Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antiviral replication with Urbani and Toronto-2 strains of live SARS-CoV, as well as cytotoxicity of selected compounds was investigated using three in vitro assays, cytopathic effect (CPE) inhibition assay, neutral red (NR) uptake assay, and virus yield reduction assay as described in Kumaki et al. (2011) (link).
For cell viability assays, cells were seeded in 96-well black tissue culture plates (Costar) and treated with compounds with final concentration of 1% DMSO. The quantity of the ATP present in metabolically active cells was determined with CellTiter-Glo® luminescent cell viability assay kits (Promega, Madison, WI).
Zhou Y., Vedantham P., Lu K., Agudelo J., Carrion R J.r., Nunneley J.W., Barnard D., Pöhlmann S., McKerrow J.H., Renslo A.R, & Simmons G. (2015). Protease inhibitors targeting coronavirus and filovirus entry. Antiviral Research, 116, 76-84.
+ Open protocol
+ Expand
2

Antiviral Screening of SARS-CoV Strains

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antiviral replication with Urbani and Toronto-2 strains of live SARS-CoV, as well as cytotoxicity of selected compounds was investigated using three in vitro assays, cytopathic effect (CPE) inhibition assay, neutral red (NR) uptake assay, and virus yield reduction assay as described in [22 (link)].
For cell viability assays, cells were seeded in 96-well black tissue culture plates (Costar) coated with compounds with final concentration of 1% DMSO. The quantity of the ATP present in metabolically active cells was determined with CellTiter-Glo® luminescent cell viability assay kits (Promega, Madison, WI).
Zhou Y., Vedantham P., Lu K., Agudelo J., Carrion R J.r., Nunneley J.W., Barnard D., Pöhlmann S., McKerrow J.H., Renslo A.R, & Simmons G. (2015). Protease inhibitors targeting coronavirus and filovirus entry. Antiviral Research, 116, 76-84.
+ Open protocol
+ Expand
3

Evaluating RPE Cell-Derived Factors on Retinal Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols
Live and dead assay was performed as previously described by Léveillard et al.7 (link) Briefly, RPE cells transduced with GFP, OTX2, and OTX2L were incubated for 1 week with CDM. Conditioned medium from RPE-transduced cells was collected and was added to primary retina cultures from chicken embryos (stage 29), prepared as described earlier,67 (link) in 96-well black tissue-culture plates (Corning) and incubated for 7 days at 37°C in 5% CO2. 14 negative control wells (conditioned medium) were also included. We used a live and dead assay (Molecular Probes) to monitor cell viability. A blind protocol was employed such that the person performing the live and dead analysis did not know which supernatant came from RPE-GFP- or RPE-OTX2-transduced cells. For acquisition and cell counting, we developed an algorithm based on the Metamorph software (Universal Imaging). We read plates under an inverted fluorescence microscope (TE 200, Nikon) equipped with a mercury epifluorescent lamp with two excitation filters (485 and 520 nm), two emission filters (520 and 635 nm), a 10× objective, a computer-driven motorized scanning stage (Märzhäuser), and a CCD camera. For the assay, we compared numbers of live cells with the mean number of live cells in the negative controls. Each assay was repeated three independent times with four replicates for each condition.
Kole C., Klipfel L., Yang Y., Ferracane V., Blond F., Reichman S., Millet-Puel G., Clérin E., Aït-Ali N., Pagan D., Camara H., Delyfer M.N., Nandrot E.F., Sahel J.A., Goureau O, & Léveillard T. (2017). Otx2-Genetically Modified Retinal Pigment Epithelial Cells Rescue Photoreceptors after Transplantation. Molecular Therapy, 26(1), 219-237.
+ Open protocol
+ Expand
4

Ethidium Bromide Efflux Assay for S. aureus

Check if the same lab product or an alternative is used in the 5 most similar protocols
The EtBr efflux inhibition assay was performed by a method described earlier (12 (link), 14 (link)). The S. aureus SA-1199B was grown at 37°C until the OD600nm reached 1.0. The culture was pelleted for 10 min at 16,060 × g, washed twice with Phosphate buffered saline (PBS; pH 7.4), and diluted to OD600 of 0.4 in PBS. Further, the bacterial suspension was loaded with EtBr (4 μg/mL), treated with compounds at sub-inhibitory concentrations (1/4 × MIC), then incubated at 25° C for 60 min to allow maximum accumulation to occur. After maximum accumulation occurred, bacteria were pelleted for 15 min at 16,060 × g and then resuspended in PBS. Finally, the aliquots were loaded to 96-well black tissue culture plates (Corning, U.S.). The fluorescence was recorded for 30 min (6 min time interval) at excitation and emission wavelength of 530 nm and 600 nm, respectively, in a microplate reader (BioTek, USA). The change in fluorescence was also determined in the presence and absence of 0.4% glucose.
Mahey N., Tambat R., Chandal N., Verma D.K., Thakur K.G, & Nandanwar H. (2021). Repurposing Approved Drugs as Fluoroquinolone Potentiators to Overcome Efflux Pump Resistance in Staphylococcus aureus. Microbiology Spectrum, 9(3), e00951-21.
+ Open protocol
+ Expand
5

Ethidium Bromide Efflux Assay for S. aureus

Check if the same lab product or an alternative is used in the 5 most similar protocols
The EtBr efflux inhibition assay was performed by a method described earlier (12 (link), 14 (link)). The S. aureus SA-1199B was grown at 37°C until the OD600nm reached 1.0. The culture was pelleted for 10 min at 16,060 × g, washed twice with Phosphate buffered saline (PBS; pH 7.4), and diluted to OD600 of 0.4 in PBS. Further, the bacterial suspension was loaded with EtBr (4 μg/mL), treated with compounds at sub-inhibitory concentrations (1/4 × MIC), then incubated at 25° C for 60 min to allow maximum accumulation to occur. After maximum accumulation occurred, bacteria were pelleted for 15 min at 16,060 × g and then resuspended in PBS. Finally, the aliquots were loaded to 96-well black tissue culture plates (Corning, U.S.). The fluorescence was recorded for 30 min (6 min time interval) at excitation and emission wavelength of 530 nm and 600 nm, respectively, in a microplate reader (BioTek, USA). The change in fluorescence was also determined in the presence and absence of 0.4% glucose.
Mahey N., Tambat R., Chandal N., Verma D.K., Thakur K.G, & Nandanwar H. (2021). Repurposing Approved Drugs as Fluoroquinolone Potentiators to Overcome Efflux Pump Resistance in Staphylococcus aureus. Microbiology Spectrum, 9(3), e00951-21.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!