For WB, Samples were loaded onto 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes (Millipore Corp, Bedford, MA, USA) and incubated with primary CRT, pEGFR1173, pEGFR-Tyr1068 (pEGFR1068) (Abcam), pEGFR-Tyr845 (pEGFR845) (Abcam), Fibronectin, Integrinβ1, Integrinα5 (Abcam), c-Myc (Cell Signaling Technology, Danvers, MA, USA), pERK (Cell Signaling Technology), Caveolin-1 (Proteintech), E-cad, N-cadherin (N-cad, Abcam), Vimentin (Proteintech), MMP9 (Proteintech), ZO-1 (Proteintech), β-catenin (Proteintech), GATA3 (Proteintech), alpha smooth muscle actin (a-SMA, Abcam) and GAPDH (Proteintech) antibodies overnight at 4 °C. Membranes were incubated with horseradish peroxidase-conjugated monoclonal secondary antibody (Santa Cruz, CA, UK) at room temperature for 1.5 h, respectively. Immunoreactive protein bands were visualized with an ECL Detection Kit (Thermo scientific, Rockford, IL, USA). Each experiment was repeated three times.
Pegfr1173
Manufactured by Abcam
Sourced in United States
PEGFR1173 is a recombinant human protein that corresponds to the extracellular domain of the epidermal growth factor receptor (EGFR). It is produced in HEK293 cells.
Automatically generated - may contain errors
Lab products found in correlation
Fibronectin, by Abcam (1 mentions)
Vimentin, by Proteintech (1 mentions)
Integrin α5, by Abcam (1 mentions)
E cad, by Abcam (1 mentions)
Gata3, by Proteintech (1 mentions)
Horseradish peroxidase conjugated monoclonal secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Caveolin 1, by Proteintech (1 mentions)
N cadherin n cad, by Abcam (1 mentions)
Alpha smooth muscle actin a sma, by Abcam (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Integrin β1, by Abcam (1 mentions)
Ecl detection kit, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
β catenin, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Gefitinib, by Thermo Fisher Scientific (1 mentions)
Paxillin, by BD (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Cilengitide, by Selleck Chemicals (1 mentions)
2 protocols using pegfr1173
1
Quantitative Western Blot Analysis
2
EGFR Signaling Pathway Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The growth factor EGF was from Life Technologies (Carlsbad, CA). Antibodies were as follows: EGFR (555996; BD Biosciences, San Jose, CA), paxillin (610052; BD Biosciences), ppaxillin Y118 (44-722G; Life Technologies), and pEGFR1173 (Abcam, Cambridge, MA). Drugs were erlotinib (LC Labs, Woburn, MA), gefitinib (Peprotech, Rocky Hill, NJ), Cilengitide (Selleck Chemicals, Houston, TX), P1D6 α5 blocking antibody (DSHB, Iowa City, IA), and PTP1Bi (Millipore, Burlington, MA).
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