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1 14c linoleic acid

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1-14C-linoleic acid is a radioactive tracer used for research purposes. It is a labeled form of the essential fatty acid linoleic acid, with the carbon-14 isotope incorporated at the first carbon position. This product is intended for use in scientific investigations, but a detailed description of its specific applications is not available within the scope of this request.

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Lab products found in correlation

1 14c palmitic acid, by PerkinElmer (2 mentions) 1 14c oleic acid, by PerkinElmer (1 mentions)

3 protocols using 1 14c linoleic acid

1

Rat Cardiomyocyte Lipid Metabolism

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Cardiac myocytes were prepared from the hearts of control and PVR pups as described [16 (link)]. The protocol allows for rat cardiomyocytes to be cultured for up to 72 h at 37°C in 5% CO2 without significant change in phenotype [17 (link)]. Enough hearts were collected to yield approximately 15-20million cardiac myocytes sufficient to plate 15–20 x 35mm dishes (1 million/dish, Corning PrimeriaTM). Isolated cardiac myocytes were incubated with 0.1 mM [1,3-3H]glycerol (2 μCi/dish, Perkin Elmer) or 0.1 μM [1-14C]linoleic acid (2 μCi/dish, Perkin Elmer) bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio) for up to 360 min (6 h) and radioactivity incorporated into phospholipids determined as previously described [18 (link)].
Cole L.K., Sparagna G.C., Dolinsky V.W, & Hatch G.M. (2022). Altered cardiolipin metabolism is associated with cardiac mitochondrial dysfunction in pulmonary vascular remodeled perinatal rat pups. PLoS ONE, 17(2), e0263520.
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2

Fatty Acid Oxidation Measurement

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Fatty acid oxidation was measured according to previous publication 37 . 1-14C-linoleic acid and 1-14C-palmitic acid were purchased from PerkinElmer. Briefly, isolated CD4+ or CD8+ T lymphocytes were pretreated with linoleic acid or kept in regular media. After 24 hrs. cell media was changed to media containing 50 μM cold linoleic acid plus 1μCi 1-14C-linoleic acid/ml or 50 μM cold palmitic acid plus 1μCi 1-14C-palmitic acid /ml. After 2 hours medium was removed and mixed with concentrated perchloric acid (final concentration 0.3M) plus BSA (final concentration 2%) to precipitate the radiolabeled fatty acids. Samples were vortexed and centrifuged (10,000x g for 10 min). Radioactivity was determined in the supernatant to measure water-soluble β-oxidation products.
Ma C., Kesarwala A.H., Eggert T., Medina-Echeverz J., Kleiner D.E., Jin P., Stroncek D.F., Terabe M., Kapoor V., ElGindi M., Han M., Thornton A.M., Zhang H., Egger M., Luo J., Felsher D.W., McVicar D.W., Weber A., Heikenwalder M, & Greten T.F. (2016). NAFLD causes selective CD4+ T lymphocyte loss and promotes hepatocarcinogenesis. Nature, 531(7593), 253-257.
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3

Fatty Acid Oxidation Measurement

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Fatty acid oxidation was measured according to previous publication 37 . 1-14C-linoleic acid and 1-14C-palmitic acid were purchased from PerkinElmer. Briefly, isolated CD4+ or CD8+ T lymphocytes were pretreated with linoleic acid or kept in regular media. After 24 hrs. cell media was changed to media containing 50 μM cold linoleic acid plus 1μCi 1-14C-linoleic acid/ml or 50 μM cold palmitic acid plus 1μCi 1-14C-palmitic acid /ml. After 2 hours medium was removed and mixed with concentrated perchloric acid (final concentration 0.3M) plus BSA (final concentration 2%) to precipitate the radiolabeled fatty acids. Samples were vortexed and centrifuged (10,000x g for 10 min). Radioactivity was determined in the supernatant to measure water-soluble β-oxidation products.
Ma C., Kesarwala A.H., Eggert T., Medina-Echeverz J., Kleiner D.E., Jin P., Stroncek D.F., Terabe M., Kapoor V., ElGindi M., Han M., Thornton A.M., Zhang H., Egger M., Luo J., Felsher D.W., McVicar D.W., Weber A., Heikenwalder M, & Greten T.F. (2016). NAFLD causes selective CD4+ T lymphocyte loss and promotes hepatocarcinogenesis. Nature, 531(7593), 253-257.
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