Commercially available primary antibodies used in this study for immunoprecipitation (IP), immunofluorescence (IF), proximity ligation assay (PLA), Western blot (WB), and Simple Western capillary system (WES) were as follows: anti-GFP (abcam, ab6556, IF & WB 1:5000, PLA 1:2000), anti-NFH (abcam, ab72996, 1:2000), anti-Actin (MP, clone C4, 08691001, WB 1:5000), anti-GAPDH (Santa Cruz Biotechnology, sc-32233, WB 1:5000), anti-ERK1/2 (Sigma, M5670, WB 1:30000), anti-β-III tubulin TUJ1 (abcam, ab18207, IF 1:1000, WB: 1:6000), anti-Dynein heavy chain (DYNC1H1, Proteintech, #12345-1-AP, PLA 1:50), anti-importin beta 1 (MBS713065, rabbit, polyclonal, 1:1000), and rabbit anti-nucleolin (ProteinTech, 10556-1-AP, 1:100 for IF).
Dync1h1
Manufactured by Proteintech
DYNC1H1 is a gene that encodes the heavy chain of the cytoplasmic dynein 1 protein. Cytoplasmic dynein 1 is a motor protein involved in the retrograde transport of various cargoes along microtubules.
Automatically generated - may contain errors
2 protocols using dync1h1
1
Antibodies for Protein Localization and Expression
2
Antibody Validation for Cellular Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Commercially available primary antibodies (ABs) used in this study for immunoprecipitation (IP), immunofluorescence (IF), proximity ligation assay (PLA), Western blot (WB) and Simple Western capillary system (WES) were: Anti-GFP (abcam, ab6556, IF & WB 1:5000, PLA 1:2000), anti-NFH (abcam, ab72996, 1:2000), anti-Actin (MP, clone C4, 08691001, WB 1:5000), anti-GAPDH (Santa Cruz Biotechnology, sc-32233, WB 1:5000), anti-ERK1/2 (Sigma, M5670, WB 1:30000), anti-β-III tubulin TUJ1 (abcam, ab18207, IF 1:1000, WB: 1:6000), anti-Dynein heavy chain (DYNC1H1, Proteintech , #12345-1-AP, PLA 1:50), antiimportin beta 1 (MBS713065, rabbit, polyclonal, 1:1000).
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