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Gsk3 β

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GSK3-β is a serine/threonine kinase that plays a key role in the regulation of various cellular processes, including metabolism, cell proliferation, and cell survival. It is involved in the Wnt signaling pathway and the insulin signaling pathway. GSK3-β is an important target for the development of therapeutic interventions in various diseases, including neurodegenerative disorders, cancer, and metabolic diseases.

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Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions) P mapk, by Cell Signaling Technology (1 mentions) Twist, by Abcam (1 mentions) Tsg101, by BD (1 mentions) P stat, by Cell Signaling Technology (1 mentions) Snail, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Odyssey clx, by LI COR (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) β catenin, by GeneTex (1 mentions) P β catenin, by GeneTex (1 mentions) Antibody for cct β, by Abcam (1 mentions) Protein a agarose, by Merck Group (1 mentions) P akt, by GeneTex (1 mentions) E cadherin, by GeneTex (1 mentions) N cadherin, by GeneTex (1 mentions) P fak, by GeneTex (1 mentions)

2 protocols using gsk3 β

1

Immunoblotting analysis of extracellular vesicles

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SDS-PAGE was used to separate equal amounts of protein (quantified by Sypro® Ruby stain) or equal volume of EV or WCL samples. Proteins were transferred to the nitrocellulose membrane (Thermo scientific), blocked with 5% skim milk and probed with the following antibodies: TSG101 (BD Transduction Laboratories: catalog number—612696), CD63 (Bio-Rad: catalog number—MCA2042GA), p16 (Cell Signaling Technologies®: catalog number—2407S), GSK3-β (Gene Tex: catalog number—GTX111192), Vimentin (Cell Signaling Technologies®: catalog number—5741S), GAPDH (Cell Signaling Technologies®: catalog number—5174S), Twist (Abcam: catalog number—ab50857), Snail (Cell Signaling Technologies®: catalog number—3879S), p-MAPK (Cell Signaling Technologies®: catalog number—9101S), p-STAT (Cell Signaling Technologies®: catalog number—9134S), p62 (Cell Signaling Technologies®: catalog number—5114S), p53 (Cell Signaling Technologies®: catalog number – 2524S) and β-actin (Cell Signaling Technology® catalog number—4970). Fluorescent conjugated rabbit and mouse secondary antibodies were used and the protein bands were visualized using ODYSSEY CLx (LI-COR®).
Samuel M., Fonseka P., Sanwlani R., Gangoda L., Chee S.H., Keerthikumar S., Spurling A., Chitti S.V., Zanker D., Ang C.S., Atukorala I., Kang T., Shahi S., Marzan A.L., Nedeva C., Vennin C., Lucas M.C., Cheng L., Herrmann D., Pathan M., Chisanga D., Warren S.C., Zhao K., Abraham N., Anand S., Boukouris S., Adda C.G., Jiang L., Shekhar T.M., Baschuk N., Hawkins C.J., Johnston A.J., Orian J.M., Hoogenraad N.J., Poon I.K., Hill A.F., Jois M., Timpson P., Parker B.S, & Mathivanan S. (2021). Oral administration of bovine milk-derived extracellular vesicles induces senescence in the primary tumor but accelerates cancer metastasis. Nature Communications, 12, 3950.
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2

Protein Co-Immunoprecipitation Assay

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Most chemicals used and Protein A-agarose were purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibody for CCT-β was purchased from Abcam (Cambridge, MA, USA) for the co-IP assay. For western blots, the specific antibodies for GAPDH (100118), CCT-α (16399), CCT-β (112283), CCT-γ (33073), CCT-δ (33536), CCT-ε (110167), CCT-ζ (105148), CCT-η (101347), CCT-θ (115466), XIAP (111202), β-catenin (101435), P-β-catenin (S675) (132611), P-β-catenin (Ser33/Ser37/Thr41) (132605), Slug (121924), Snail (100754), Twist (127310), E-cadherin (100443), MUC1 (100459), N-cadherin (112734), MMP2 (104577), MMP9 (100458), AKT (121973), P-AKT (128414), GSK-3β (111192), P-GSK-3β (50090), Survivin (100052), NF-kB p65 (102090), fibronectin (112794), β1 (112971), Src (134412), P-Src (54701), FAK (50489), and P-FAK (129840) were from GeneTex (Irvine, CA, USA). The catalog numbers are in parentheses.
Chang Y.X., Lin Y.F., Chen C.L., Huang M.S., Hsiao M, & Liang P.H. (2020). Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways. Cancers, 12(12), 3865.
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