Log In Sign up
The largest database of trusted experimental protocols

Spi1 pu 1

Manufactured by Cell Signaling Technology
Visit Supplier

SPI1/PU.1 is a lab equipment product offered by Cell Signaling Technology. It is a transcription factor that plays a critical role in the development and function of hematopoietic cells. The core function of SPI1/PU.1 is to regulate the expression of genes involved in cell differentiation, proliferation, and survival.

Automatically generated - may contain errors

Lab products found in correlation

Jarid2, by Cell Signaling Technology (2 mentions) Ship1 inpp5d, by BioLegend (2 mentions) Uqcrfs1, by LifeSpan BioSciences (2 mentions) Lin28b, by Cell Signaling Technology (2 mentions) H2ak119ub, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) Gapdh, by Biosynth (1 mentions) Protease complete, by Roche (1 mentions)

5 protocols using spi1 pu 1

1

Immunoblotting antibodies for cell biology

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunoblotting: SHIP1 (Inpp5d) (BioLegend, #656601), PU.1 (Spi1) (Cell Signaling, #2258S), Tab2 (LifeSpan, #LS-C498046), Jarid2 (Cell Signaling, #13594T), Uqcrfs1 (LifeSpan, #LS-C185466), HIF-1α (Hif1a) (SIGMA, #ABE279), and β-Actin (Actb) (Sigma, #A3853) antibodies. AID (Aicda) specific antibody is a gift from J. Chaudhuri (MSKCC).
Hsin J.P., Lu Y., Loeb G.B., Leslie C.S, & Rudensky A.Y. (2018). The effect of cellular context on miR-155-mediated gene regulation in four major immune cell types. Nature immunology, 19(10), 1137-1145.
+ Open protocol
+ Expand
2

Immunoblotting antibodies for cell biology

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunoblotting: SHIP1 (Inpp5d) (BioLegend, #656601), PU.1 (Spi1) (Cell Signaling, #2258S), Tab2 (LifeSpan, #LS-C498046), Jarid2 (Cell Signaling, #13594T), Uqcrfs1 (LifeSpan, #LS-C185466), HIF-1α (Hif1a) (SIGMA, #ABE279), and β-Actin (Actb) (Sigma, #A3853) antibodies. AID (Aicda) specific antibody is a gift from J. Chaudhuri (MSKCC).
Hsin J.P., Lu Y., Loeb G.B., Leslie C.S, & Rudensky A.Y. (2018). The effect of cellular context on miR-155-mediated gene regulation in four major immune cell types. Nature immunology, 19(10), 1137-1145.
+ Open protocol
+ Expand
3

Western Blot Analysis of SPI1/PU.1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was homogenized in RIPA buffer; phosphatase (Sigma) and protease (Complete®, Roche) inhibitors were added. Lysates were resolved by SDS–PAGE and immunoblotted for SPI1/PU.1 (Cell Signaling Technology, Leiden, the Netherlands) overnight at 4°C. GAPDH (Fitzgerald Industries, Huissen, the Netherlands) was used as a loading control. Imaging was performed on a ChemiDoc XRS+ System (BioRad, Temse, Belgium). Quantification was done using Image J software (NIH).
Van Aelst L.N., Summer G., Li S., Gupta S.K., Heggermont W., De Vusser K., Carai P., Naesens M., Van Cleemput J., Van de Werf F., Vanhaecke J., Thum T., Waer M., Papageorgiou A.‐., Schroen B, & Heymans S. (2015). RNA Profiling in Human and Murine Transplanted Hearts: Identification and Validation of Therapeutic Targets for Acute Cardiac and Renal Allograft Rejection. American Journal of Transplantation, 16(1), 99-110.
+ Open protocol
+ Expand
4

Antibody-based Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: CBX2 (Abcam 80,044 or Bethyl A302–524A), LIN28B (Cell Signaling Technologies 11,965, clone D4H1), SPI1/PU.1 (Cell Signaling Technologies 2258), H2AK119Ub (Cell Signaling Technologies 8240) and β-actin (Cell Signaling Technologies 4970). Western blots were quantified with ImageJ software.
Wang D., Tanaka-Yano M., Meader E., Kinney M.A., Morris V., Lummertz da Rocha E., Liu N., Liu T., Zhu Q., Orkin S.H., North T.E., Daley G.Q, & Rowe R.G. (2022). Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis. Cell reports, 39(1), 110587.
+ Open protocol
+ Expand
5

Antibody-based Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: CBX2 (Abcam 80,044 or Bethyl A302–524A), LIN28B (Cell Signaling Technologies 11,965, clone D4H1), SPI1/PU.1 (Cell Signaling Technologies 2258), H2AK119Ub (Cell Signaling Technologies 8240) and β-actin (Cell Signaling Technologies 4970). Western blots were quantified with ImageJ software.
Wang D., Tanaka-Yano M., Meader E., Kinney M.A., Morris V., Lummertz da Rocha E., Liu N., Liu T., Zhu Q., Orkin S.H., North T.E., Daley G.Q, & Rowe R.G. (2022). Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis. Cell reports, 39(1), 110587.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!