Coomassie blue

Cysteamine was purchased from Solarbio Technology Co., Ltd. (Beijing, China), and the drug was dissolved in embryo culture medium (salinity: 0.5%, CaCO₃: 150 mg/L, pH: 7.0, conductivity: 500 μS/cm). Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), reactive oxygen species (ROS) detection reagents, and Coomassie Blue were purchased from Nanjing Jiancheng Bioengineering Institute. RNAiso Plus was purchased from Takara Bio Inc. (Shiga, Japan). cDNA reverse transcription kit and qPCR kit were purchased from Beijing Quanjin Biological Co., Ltd. (Beijing, China). Sodium Valproate was purchased from MedchemExpress (New Jersey, United States).

Methyl parathion (CAS: 298-00-0, purity > 98%) was purchased as a solid from the Beijing North Weiye Metrology Technology Institute (Beijing, China), dissolved in pure water, and prepared as working solutions of 2.5, 5, and 10 mg/L prior to the experiments. Acridine orange (CAS: 10127-02-3) in this study was purchased from Aladdin Reagent (Shanghai, China) Co., Ltd. Astaxanthin (analytical standard, purity > 98%, CAS: 472-61-7) was purchased from Beijing Solarbio Science and Technology Co., Ltd. Kits for the evaluation of enzyme activity or other biological indicators such as CAT, SOD, ROS, MDA, Coomassie blue (for determination of total protein concentration), and Trizol reagents were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Reverse transcriptase kits and SYBR green reagents were provided by Takara (Dalian, China). All other biochemical reagents were purchased from Aladdin Reagent (Shanghai, China) Co., Ltd. and were of analytical quality.