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Hplc formic acid

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HPLC formic acid is a chemical reagent used in high-performance liquid chromatography (HPLC) analysis. It is a mobile phase additive that helps improve the separation and resolution of analytes during HPLC runs.

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Lab products found in correlation

Hplc water, by Merck Group (2 mentions) Thermo ultimate 3000, by AB Sciex (2 mentions) Hplc grade acetone, by Lach-Ner (2 mentions) Tripletof 5600 mass spectrometer, by AB Sciex (2 mentions) Lc ms grade water, by Merck Group (2 mentions) Lc ms grade methanol, by Merck Group (2 mentions) Apomorphine, by Cayman Chemical (1 mentions) Nexera uplc system, by Shimadzu (1 mentions) Psoralen, by Chengdu Alfa Biotechnology (1 mentions)

4 protocols using hplc formic acid

1

Quantification of CuET in HPLC-ESI-QTOF

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The HR-MRM analysis was performed on HPLC-ESI-QTOF system consisting of HPLC chromatograph Thermo UltiMate 3000 with AB Sciex TripleTOF 5600+ mass spectrometer, using the DuoSpray ESI source operated at ion source voltage 5500 V, ion source gas flow rates 40 units, curtain gas flow rate 30 units, declustering potential 100 V and temperature 400°C. Data were acquired in Product ion mode with two parent masses 358.9 and 360.9 for analysis of CuET. Chromatographic separation was done by PTFE column especially designed for analysis of strong metal chelators filled by C18 sorbent (IntellMed, cat.no.IM_301). Analysis was performed at room temperature and flow rate 1500 μL/min with isocratic chromatography. Mobile phase consisted of HPLC grade acetone (Lachner) 99.9%, HPLC water (Merck Millipore) 0.1% and 0.03% HPLC formic acid (Sigma). Acquired mass spectra were evaluated in software PeakView 1.2, where extracted ion chromatograms of transitions 88.0 and 116.0 (common for both parent masses) with 0.1 mass tolerance was Gaussian smoothened with width of 2 points. Peak area was then recorded and recalculated to ng/ml according to calibration curve.
Skrott Z., Mistrik M., Andersen K.K., Friis S., Majera D., Gursky J., Ozdian T., Bartkova J., Turi Z., Moudry P., Kraus M., Michalova M., Vaclavkova J., Dzubak P., Vrobel I., Pouckova P., Sedlacek J., Miklovicova A., Kutt A., Li J., Mattova J., Driessen C., Dou Q.P., Olsen J., Hajduch M., Cvek B., Deshaies R.J, & Bartek J. (2017). Alcohol-abuse drug disulfiram targets cancer via p97 segregase adapter NPL4. Nature, 552(7684), 194-199.
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2

LCMS Quantification of Nuciferine and Apomorphine

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LCMS-grade methanol, LCMS-grade water, and HPLC-formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nuciferine and apomorphine were purchased from Cayman Chemical (Ann Arbor, MI, USA). Stock solutions of each standard at a concentration of 10 ppm were prepared by diluting the powder in methanol. Nuciferine and apomorphine were quantified using a NEXERA UPLC system (Shimadzu Corp., Kyoto, Japan) equipped with a mass spectrometer (Triple quadrupole, LCMS8060, Shimadzu, Kyoto, Japan) as previously described [18 (link),25 (link)]. The detection was completed in multiple reaction monitoring mode (MRM) (Table 5). Samples were run in triplicate with external standards in between and the injection volume was 1 μL. The acquired chromatographic results were processed in LabSolutions Insight software version 3.2 (Shimadzu). For each compound, calibration curves (0.005–0.1 ppm) were created by linking the peak area and the concentration.
Dosoky N.S., Shah S.A., Dawson J.T., Banjara S.S., Poudel A., Bascoul C, & Satyal P. (2023). Chemical Composition, Market Survey, and Safety Assessment of Blue Lotus (Nymphaea caerulea Savigny) Extracts. Molecules, 28(20), 7014.
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3

Quantification of CuET in HPLC-ESI-QTOF

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HR-MRM analysis was performed on HPLC-ESI-QTOF system consisting of HPLC chromatograph Thermo UltiMate 3000 with AB Sciex TripleTOF 5600+ mass spectrometer, using the DuoSpray ESI source operated at ion source voltage 5500 V, ion source gas flow rates 40 units, curtain gas flow rate 30 units, declustering potential 100 V and temperature 400°C. Data were acquired in Product ion mode with two parent masses 358.9 and 360.9 for analysis of CuET. Chromatographic separation was done by PTFE column especially designed for analysis of strong metal chelators filled by C18 sorbent (IntellMed, cat.no.IM_301). Analysis was performed at room temperature and flow rate 1500 μL/min with isocratic chromatography. Mobile phase consisted of HPLC grade acetone (Lachner) 99.9%, HPLC water (Merck Millipore) 0.1% and 0.03% HPLC formic acid (Sigma). Acquired mass spectra were evaluated in software PeakView 1.2, where extracted ion chromatograms of transitions 88.0 and 116.0 (common for both parent masses) with 0.1 mass tolerance was Gaussian smoothened with width of 2 points. Peak area was then recorded and recalculated to ng/ml according to calibration curve.
Skrott Z., Mistrik M., Andersen K.K., Friis S., Majera D., Gursky J., Ozdian T., Bartkova J., Turi Z., Moudry P., Kraus M., Michalova M., Vaclavkova J., Dzubak P., Vrobel I., Pouckova P., Sedlacek J., Miklovicova A., Kutt A., Li J., Mattova J., Driessen C., Dou Q.P., Olsen J., Hajduch M., Cvek B., Deshaies R.J, & Bartek J. (2017). Alcohol-abuse drug disulfiram targets cancer via p97 segregase adapter NPL4. Nature, 552(7684), 194-199.
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4

Compound Standards for Analytical Methods

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Xanthotoxin, herniarin, toncarine, bergamottin, oxypeucedanin, biacangelicol, psoralen, isopimpinellin, bergapten, and imperatorin (purity ≥ 98%) were purchased from Chengdu Alfa Biotechnology (Chengdu, China). 5-Geranyloxy-7-methoxycoumarin (purity ≥ 99%) was bought from Extrasynthese (Genay, France). Trioxsalen and 6′,7′-epoxybergamottin (purity ≥ 98%) were obtained from Cayman Chemical Company (Michigan, USA). Citropten (purity ≥ 99%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). LCMS-grade methanol, LCMS-grade water, and HPLC-formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Stock solutions of each standard at a concentration of 10 ppm were prepared by diluting the powder in methanol.
Dosoky N.S., Satyal P, & Setzer W.N. (2022). Authentication of Citrus spp. Cold-Pressed Essential Oils by Their Oxygenated Heterocyclic Components. Molecules, 27(19), 6277.
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