Mice were sacrificed by CO2 asphyxiation. Blood samples were drawn from the inferior vena cava and collected in heparin-coated microcentrifuge tubes. Plasma was separated by centrifugation at 3000 rpm at 4 °C for 10 min. Whole liver was isolated from each mouse and weighed. Plasma and liver samples were kept at − 80 ºC until further assay. The levels of total cholesterol and triglyceride in plasma and liver were tested with enzymatic methods by cholesterol assay kit and triglyceride assay kit respectively (Stanbio Laboratory, USA) according to the manufacturer’s instructions. The levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured using the HDL-C and LDL-C assay kits (Nanjing Jiancheng Bioengineering Institute, China). Plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by ALT and AST assay kits (Nanjing Jiancheng Bioengineering Institute).
Hdl c and ldl c assay kits
Manufactured by Nanjing Jiancheng
Sourced in China
The HDL-C and LDL-C assay kits are laboratory equipment used for the quantitative determination of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in human serum or plasma samples. These kits provide a method for the direct and selective measurement of HDL-C and LDL-C levels.
Automatically generated - may contain errors
2 protocols using hdl c and ldl c assay kits
1
Assessing Lipid and Liver Profiles in Mice
2
Lipid and Enzyme Analysis in Mice
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Mice were sacri ced by CO 2 asphyxiation. Blood samples were drawn from the inferior vena cava and collected in heparin-coated microcentrifuge tubes. Plasma was separated by centrifugation at 3000 rpm at 4°C for 10 min. Whole liver was isolated from each mouse and weighed. Plasma and liver samples were kept at -80 ºC until further assay. The levels of total cholesterol and triglyceride in plasma and liver were tested with enzymatic methods by cholesterol assay kit and triglyceride assay kit respectively (Stanbio Laboratory, USA) according to the manufacturer's instructions. The levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured using the HDL-C and LDL-C assay kits (Nanjing Jiancheng Bioengineering Institute, China). Plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by ALT and AST assay kits (Nanjing Jiancheng Bioengineering Institute).
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