3.0 mm zirconium beads

Manufactured by Benchmark Scientific

Sourced in United States

The 3.0 mm zirconium beads are a type of laboratory equipment used for various applications. They are composed of zirconium, a durable and chemically resistant material. These beads are designed to provide an efficient means of homogenizing, disrupting, or grinding samples in a variety of laboratory processes.

Automatically generated - may contain errors

Lab products found in correlation

Chlorogenic acid, by ChromaDex (1 mentions) Beadbug microtube homogenizer, by Benchmark Scientific (1 mentions) Quercetin 3 glucoside, by Merck Group (1 mentions) Sinapic acid, by Merck Group (1 mentions) Caffeic acid, by Merck Group (1 mentions) Hippuric acid, by Merck Group (1 mentions) Bca assay kit, by Thermo Fisher Scientific (1 mentions) Fastprep 24 5g instrument, by MP Biomedicals (1 mentions)

Close spelling variants of this product

3.0 mm beads Zirconium beads

2 protocols using 3.0 mm zirconium beads

Characterization of Red Leaf Lettuce

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following standards were purchased: sinapic acid, hippuric acid, caffeic acid, quercetin 3-glucoside (all ≥98% purity, Sigma, St. Louis, MO), chlorogenic acid (primary standard grade, ≥98% purity, Chromadex, Irvine, CA), and cyanidin 3-O-β-glucopyranoside (≥97% purity, Polyphenols, Norway). BeadBug™ Microtube Homogenizer and 3.0 mm zirconium beads were purchased from Benchmark Scientific (Edison, NJ).
RSL looseleaf variety NFR, was provided by Shamrock Seed Co. (Salinas, CA) and green leaf lettuce (GL) variety Crispa (Andy Boy, Salinas, CA) were purchased from the grocery store (Stop and Shop, Highland Park, NJ). NFR plant and seed vouchers were made and deposited at the Chrysler Herbarium and ATCC [4 (link),12 (link)]. Outer leaves and tops of RSL lettuce heads were used as they have the highest levels of phenolics. Lettuce leaves were rinsed, lyophilized and homogenized in a Vitamix Professional 500 Blender (Cleveland, OH) to a fine powder. Dried RSL and GL powders were used for phytochemical extraction, nutritional analyses, and rodent diet formulations.

Cheng D.M., Roopchand D.E., Poulev A., Kuhn P., Armas I., Johnson W.D., Oren A., Ribnicky D., Zelzion E., Bhattacharya D, & Raskin I. (2016). High Phenolics Rutgers Scarlet Lettuce Improves Glucose Metabolism in High Fat Diet-Induced Obese Mice. Molecular nutrition & food research, 60(11), 2367-2378.

+ Open protocol

+ Expand

Cytokine Profiling of Breast Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen breast tissue was placed into chilled 2-ml homogenization tubes containing 3.0 mm zirconium beads (Benchmark Scientific, Edison, NJ, USA). Cold PBS with 2X complete protease inhibitor cocktail (Roche, Basel, Switzerland) was added in a ratio of 4 μl/mg (tumor) or 3 μl/mg (non-tumor) tissue, and samples were homogenized using a FastPrep-24 5G instrument (MP Biomedicals) with 3 runs of 30 s at 6 m/s. After sequential centrifugation (14,000×g for 15 min at 4 °C, transfer to a new tube, then 14,000×g for 10 min at 4 °C), supernatant aliquots were taken for protein quantification using a BCA assay kit (Thermo Scientific) in accordance with the manufacturer’s protocol. PBS with protease inhibitor was added to normalize the protein concentrations of all supernatant samples to 1.5 mg/ml, and samples were stored at − 80 °C until analysis. Cytokine expression was evaluated using the Human Cytokine/Chemokine Array 65-Plex Assay performed by Eve Technologies (Calgary, AB, Canada). Background fluorescence was subtracted from all samples, and data were log₁₀-transformed prior to further analysis.

Tzeng A., Sangwan N., Jia M., Liu C.C., Keslar K.S., Downs-Kelly E., Fairchild R.L., Al-Hilli Z., Grobmyer S.R, & Eng C. (2021). Human breast microbiome correlates with prognostic features and immunological signatures in breast cancer. Genome Medicine, 13, 60.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!