Cx3cr1gfp/+ mice were infected with 2x108 Yptb-OVA, and on day 6 post infection LP cells were isolated from the ileum and cecum. Cells were stained with antibodies for CD11c, CD11b, I-Ab and dump gate (CD103, IgA (AD3, LSBio), CD19 (1D3, eBioscience), TCRβ (H57-597, eBioscience), TCRγδ (GL3, eBioscience)) sorted using a FACSAria (BD) to greater than 96% purity. Congenically marked CD8+CD44hi memory cells were sorted from Yptb-OVA memory mice and labelled with CFSE. 5x104 APCs and 5x104 memory T cells were mixed and incubated for 3 days with the addition of 5U/ml IL-2 for the final day of incubation. CFSE dilution was examined by flow cytometry and a live/dead stain was used to exclude dead cells from the analysis. Yptb- OVA memory cells were also incubated with APCs pulsed with SIINFEKL and YopE69-77 peptides to determine the maximum antigen specific response, and 60-70% of sorted Yptb-OVA memory cells divided in response to this peptide mixture.
Iga ad3
Manufactured by LSBio
The IgA (AD3) is a lab equipment product that is used for the detection and quantification of immunoglobulin A (IgA) in biological samples. IgA is an important antibody that plays a role in mucosal immunity. The core function of this product is to provide a tool for researchers and clinicians to analyze IgA levels in their studies or diagnostic procedures.
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2 protocols using iga ad3
1
Antigen-specific CD8+ T cell responses
2
Antigen-specific CD8+ T cell responses
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Cx3cr1gfp/+ mice were infected with 2x108 Yptb-OVA, and on day 6 post infection LP cells were isolated from the ileum and cecum. Cells were stained with antibodies for CD11c, CD11b, I-Ab and dump gate (CD103, IgA (AD3, LSBio), CD19 (1D3, eBioscience), TCRβ (H57-597, eBioscience), TCRγδ (GL3, eBioscience)) sorted using a FACSAria (BD) to greater than 96% purity. Congenically marked CD8+CD44hi memory cells were sorted from Yptb-OVA memory mice and labelled with CFSE. 5x104 APCs and 5x104 memory T cells were mixed and incubated for 3 days with the addition of 5U/ml IL-2 for the final day of incubation. CFSE dilution was examined by flow cytometry and a live/dead stain was used to exclude dead cells from the analysis. Yptb- OVA memory cells were also incubated with APCs pulsed with SIINFEKL and YopE69-77 peptides to determine the maximum antigen specific response, and 60-70% of sorted Yptb-OVA memory cells divided in response to this peptide mixture.
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