Anti sca 1 pe

The mice were subjected to cervical dislocation following anesthesia with isoflurane. The mice were then sterilized with 70% ethanol for 5 min. Mouse BMMSC were isolated from the femurs and tibias of wild‐type or KAT2A^+/− C57BL/6 mice (male, 6–8 weeks, 20–30 g). The femurs and tibias were dissected and cleaned of connective tissue. Both ends of the femurs and tibias were incised, and the bone marrow cells were washed out with PBS, and filtered through a 70‐µm cell strainer. The collected cells were centrifuged at 1500 rpm for 5 min, resuspended in a complete MesenCult expansion medium (STEMCELL, # 05513) containing 10% FBS, 100 IU mL⁻¹ penicillin, and 100 IU mL⁻¹ streptomycin, and seeded into 25 cm² flasks and cultured at 37°C in an atmosphere with 5% CO₂. The nonadherent cells were removed after 3 days, and half of the medium was refreshed every 3 days. At 90% confluence, the cells were passaged using 0.05% trypsin. The phenotypes of the expanded mouse BMMSC were detected by flow cytometry analysis. BMMSC were harvested, washed with PBS, and incubated with anti‐CD45‐PE (BioLegend, #103105), anti‐CD11b‐FITC (BioLegend, #101205), anti‐CD34‐PE (BioLegend, #119307), anti‐CD29‐FITC (BioLegend, #102205), anti‐CD105‐FITC (Invitrogen, #MA5‐17945), anti‐Sca1‐PE (BioLegend, #108107) and anti‐CD44‐PE (BioLegend, #103023), and analyzed by flow cytometry.