HUVECs were lysed with lysis buffer RIPA (G2002; Wuhan Goodbio Biotechnology Co., Ltd., Wuhan, China). Protein quantification was conducted using the Pierce BCA protein assay kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The following primary antibodies were used: Cleaved-caspase-3 (9664), caspase-3 (9665), B-cell lymphoma 2-associated X protein (Bax; 2722), and Bcl-2 (2870) (all from Cell Signaling Technology, Inc., Danvers, MA, USA), TLR4 (sc-10741; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), p-TAK1 (4508), p-TBK1 (5483), p-p65 (3033), TAK1 (5206), TBK1 (3013), p65 (8242), and GAPDH (2118) (all from Cell Signaling Technology, Inc.). The corresponding peroxidase-conjugated secondary antibodies used were IRdye 800CW-conjugated goat anti-mouse immunoglobulin G (926–32210) and IRDye 800CW-conjugated goat anti-rabbit immunoglobulin G (926–32211) (both from LI-COR Biosciences, Lincoln, NE, USA). The blots were scanned using a two-color infrared imaging system (Odyssey; LI-COR Biosciences) and the expression of target proteins were normalized to the expression of GAPDH.
Bcl 2 2870
Manufactured by Cell Signaling Technology
Sourced in United States
Bcl-2 (2870) is a recombinant rabbit monoclonal antibody that recognizes human Bcl-2 protein. Bcl-2 is a key regulator of apoptosis, playing a crucial role in cell survival and death. This antibody can be used for the detection of Bcl-2 in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Gapdh, by LI COR (1 mentions)
Gapdh 2118, by Cell Signaling Technology (1 mentions)
Irdye 800cw conjugated goat anti rabbit immunoglobulin g, by LI COR (1 mentions)
Odyssey, by LI COR (1 mentions)
Antifoam, by Eli Lilly (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions)
Vs 5584, by Selleck Chemicals (1 mentions)
Bcl xl 2762, by Cell Signaling Technology (1 mentions)
Sx 1292 oral vehicle, by Eli Lilly (1 mentions)
Abt 737, by Selleck Chemicals (1 mentions)
2 protocols using bcl 2 2870
1
Apoptosis Signaling Pathway Regulation
2
In Vitro and In Vivo Evaluation of VS-5584 and ABT-737
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VS-5584 and ABT-737 were obtained from Selleck (Shanghai, China). Both compounds were dissolved in DMSO and stored at -20° for in vitro studies. For in vivo studies, both agents were dissolved in the SX-1292 oral vehicle [1% sodium carboxymethyl cellulose, 0.5% sodium lauryl sulfate (SLS), and 0.05% antifoam; Eli Lilly, Shanghai, China] and administered by gastric lavage [13 (link),14 (link)]. Antibodies of p-AKT (Ser 473, #9271), p-AKT (Thr 308, #9275), p-S6 ribosomal protein (S6, Ser 235/236, #2211), S6 (#2317), p-p70 S6 kinase 1 (S6K1, Thr 398, #9209), S6K1 (#2708), Bcl-2 (#2870), Bcl-xL (#2762) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #2118) were obtained from Cell Signaling Technology (Danvers, MA). The concentration of primary antibodies utilized in this study was 1: 1,000, except for p-S6 (1: 10,000) and GAPDH (1: 10,000). The caspase inhibitors z-VAD-fmk and z-DVED-fmk were obtained from Calbiochem (Shanghai, China).
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