Smz 1500 stereoscopic zoom microscope

Adult scorpionflies were caught with collecting nets, and preserved in 95% ethanol or pinned as permanent collections. The specimens examined are deposited in the Entomological Museum, Northwest A&F University, Yangling (NWAU) and the Institute of Zoology, Chinese Academy of Sciences, Beijing (IZAS). Specimens were observed under a Nikon SMZ 1500 Stereoscopic Zoom microscope. Measurements of right wings were made with a vernier caliper. The lengths of wings were measured from the base to the apex, and widths from the ending of M₄ to the costal margin vertically. Photographs were taken with a Nikon D7000 digital camera except Figure 1B with a Nikon D7100 digital camera. All pictures were further adjusted and assembled with Adobe Photoshop CS4.
Terminology follows Byers (1989) , Hua et al. (2018) (link) and Wang and Hua (2019) (link). The following abbreviations and acronyms are applied: A1, first abdominal segment (and so forth for other segments); T1, first tergum (and so forth for other segments); FL, forewing length; FW, forewing width; HL, hindwing length; and HW, hindwing width.

Specimens were collected from the Qinling and Minshan mountains in central China (Fig. 1), and deposited in the Entomological Museum, Northwest A&F University, China (NWAU). Specimens were dissected under a Nikon SMZ 1500 Stereoscopic Zoom microscope. Genitalia were macerated in cold 5% NaOH solution for 3 min and rinsed with distilled water. Wings were measured with a vernier calliper. Adult photographs were taken with a Nikon D7100 digital camera, other images were taken using a scientific digital micrography system, ZEISS SteREO Discovery.V20 equipped with an auto-montage imaging system AxioCam IC. The distribution map was constructed using ArcGIS v10.2 and Adobe Illustrator CC. All photographs were assembled with Adobe Photoshop CS6.
Terminology follows Gao et al. (2016) (link), Hua et al. (2018) (link) and Wang et al. (2019) (link). The following abbreviations and acronyms are applied: A1, first abdominal segment (and so forth for other segments); T1, first tergum (and so forth for other segments).

Zebrafish (D. rerio) wild type AB eggs were obtained from a culture maintained at the Department of Biology, University of Aveiro (Aveiro, Portugal). Zebrafish adults were kept in a recirculating system with reverse osmosis and activated carbon filtered tap water, complemented with instant ocean synthetic salt automatically adjusted for pH and conductivity. The organisms were maintained at 26.0 ± 1°C, under a 16:8 h light/ dark photoperiod cycle, with conductivity at 750 ± 50 µS/cm, pH at 7.5 ± 0.5, salinity of 0.35 and dissolved oxygen at 95% saturation. Adult fishes were fed daily with commercially artificial diet, Gemma Micro 500 (Skretting^®, Burgos, Spain). Reproduction groups of zebrafish adults were placed in aquarium with marbles in the bottom, in the afternoon of the day before the collection of the eggs. Two hours after the beginning of the illumination in the next morning, the eggs were collected and cleaned from residues. Zebrafish eggs with normal development were selected for the toxicity test, using a SMZ 1500 Stereoscopic Zoom Microscope (Nikon, Amsterdam, The Netherlands). Unfertilized, irregular or injured eggs were discarded.