Enterprises, Mumbai. Span 60,
cholesterol, disodium hydrogen phosphate, potassium dihydrogen phosphate,
ammonium acetate buffer, and mannitol were purchased from Loba Chemie,
Mumbai. Chloroform and acetonitrile were procured from Merck Pvt.,
Ltd. All solvents used were of HPLC grade, and the ingredients and
reagents used were of analytical grade. Distilled water was used for
all of the experiments.