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4 protocols using potassium dihydrogen phosphate

1

Irbesartan Formulation Development

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Irbesartan was procured from Yucca
Enterprises, Mumbai. Span 60,
cholesterol, disodium hydrogen phosphate, potassium dihydrogen phosphate,
ammonium acetate buffer, and mannitol were purchased from Loba Chemie,
Mumbai. Chloroform and acetonitrile were procured from Merck Pvt.,
Ltd. All solvents used were of HPLC grade, and the ingredients and
reagents used were of analytical grade. Distilled water was used for
all of the experiments.
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2

Extraction and Characterization of Natural Gums

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Isabgol husk and gum katira were procured from local market. Other chemicals and regents such as potassium dihydrogen phosphate, sodium hydroxide, and hydrochloric acid of analytical grade were procured from Loba Chemie, Mumbai, and used as such without further purification and modification.
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3

Evaluation of Quercetin and DCFDA Effects

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Quercetin hydrate (≥95%) and 2′,7′-dichlorodihydrofluoresceindiacetate (DCFDA, ≥97%) were procured from Merck, India. Roswell Park Memorial Institute (RPMI-1640) medium, Dulbecco’s Modified Eagle Medium (DMEM), antibiotics, fetal bovine serum, hematoxylin, and eosin were purchased from Himedia Ltd., India. All other reagents, including Sodium chloride, Potassium chloride, Disodium hydrogen phosphate, and Potassium dihydrogen phosphate, were purchased from Loba Chemie, India, and were of analytical grade.
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4

Antifungal Topical Formulation Development

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Fluconazole (Macklin, China), HPMC E5 LV, methyl paraben, propyl paraben, hydrochloric acid, potassium dihydrogen phosphate (Loba Chemie, India), xanthan gum (Titan Biotech, India), polyethylene sorbitan monooleate (tween 80), clove oil (Molychem, India), sorbitan monolaurate (span 20) (Acros Organics, USA), cinnamon leaf oil (Deo Organics, Sri Lanka), palm olein (raffles oil, Nigeria), methanol (BDH, UK), Strat-M™ membrane, 0.45 μm (Millipore, UK), filter units (0.45 μm), Candida albicans (clinical isolate grown at 37 °C for 24 h on Sabouraud dextrose agar), and deionised water.
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